Fourier transform infrared microscopy of calcified turkey leg tendon.
Fourier transform infrared microscopy of calcified turkey leg tendon.
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钙化火鸡腿腱的傅里叶变换红外显微镜。
DOI：
10.1007/bf02509541
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发表时间：
1996
影响因子：
4.2
通讯作者：
Boskey,AL
中科院分区：
文献类型：
--
作者：
Gadaleta,SJ;Camacho,NP;Mendelsohn,R;Boskey,AL
傅里叶变换红外显微镜（FT-IRMS）被用来监测钙化火鸡腱中矿物相的质量和数量的空间变化。通过分析肌腱不同区域内的50 μm×50μm区域生成光谱图。光谱的过渡区，非矿化基质结束和矿化基质开始，揭示了显着的变化，在光谱确定的矿物-基质比，而更深的矿化前沿的区域表现出相对恒定的比例。由于EDTA-脱矿基质的光谱与非矿化基质的光谱相似，因此肌腱的非矿化区域用于光谱相减。矿物ν1，ν 3磷酸盐区域（900-1200 cm−1）的宽而相对无特征的轮廓在不同的矿化阶段只显示出细微的变化。这些光谱的二阶导数进行了计算，并与那些合成的，结晶不良的羟基磷灰石（HA）。在过渡区附近矿物的二阶导数光谱中观察到的峰值位置在最不成熟的合成HA的±2 cm− 1范围内，而在矿化前沿更深处矿物的光谱在最成熟的合成HA的±2 cm− 1范围内。对腱中相同位置的横截面和纵截面的光谱以及偏振FT-IRMS数据进行分析，以确定矿物取向对用于表征矿物的参数的影响。腱的横截面和纵截面的光谱显示，ν1，ν 3磷酸盐区域或酰胺I，II或III组分（1200-1800 cm−1）没有重大差异。然而，偏振FT-IR光谱显示在这两个地区的显着差异。尽管存在这些差异，但对ν1、ν 3区域的二阶导数分析显示，用于表征结晶不良HA中磷酸根离子环境的潜在条带的位置没有显著变化。本研究的结果表明，FT-IRMS监测钙化组织中矿物相的空间变化的能力。此外，偏振辐射的掺入提供了能够评估矿物相相对于胶原基质的分子取向的方法。
Fourier transform infrared microscopy (FT-IRMS) was used to monitor spatial variations in the quality and quantity of the mineral phase in calcified turkey tendon. Spectral maps were generated by analysis of 50 μm×50μm areas within different regions of the tendon. Spectra of the transitional region, where nonmineralized matrix ends and mineralized matrix begins, revealed marked changes in the spectrally determined mineral-to-matrix ratio, whereas regions deeper into the mineralization front showed a relatively constant ratio. Since spectra of EDTA-demineralized matrix were similar to those of nonmineralized matrix, the nonmineralized regions of the tendon were used for spectral subtraction. The broad, relatively featureless contour of the mineral ν1, ν3phosphate region (900–1200 cm−1) showed only subtle changes at different stages of mineralization. Second derivatives of these spectra were calculated and compared with those of synthetic, poorly crystalline hydroxyapatite (HA). The peak positions seen in second-derivative spectra of the mineral near the transitional region were within ±2 cm−1of the least mature synthetic HAs whereas spectra of the mineral deeper into the mineralization front were within ±2 cm−1of the most mature synthetic HAs. Spectra from cross-and longitudinal sections at equivalent positions in the tendon, and polarized FT-IRMS data were analyzed to determine the effect of mineral orientation on the parameters used to characterize the mineral. Spectra of cross-aand longitudinal sections of the tendon showed no major differences in either the ν1, ν3phosphate region or the amide I, II, or III components (1200–1800 cm−1). However, polarized FT-IR spectra revealed dramatic differences in both of these regions. Despite these differences, second-derivative analysis of the ν1, ν3regions revealed no significant changes in the positions of the underlying bands used to characterize the environments of the phosphate ion in poorly crystalline HA. The results of this study demonstrate the power of FT-IRMS to monitor spatial variations of the mineral phase in calcified tissue. Also, the incorporation of polarized radiation provides a method capable of assessing the molecular orientation of the mineral phase relative to the collagen matrix.