Cyto- and histocompatibility of multilayered DNA-coatings on titanium.
Cyto- and histocompatibility of multilayered DNA-coatings on titanium.
复制标题
钛上多层 DNA 涂层的细胞和组织相容性。
DOI：
10.1002/jbm.a.30583
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
J. Jansen
中科院分区：
文献类型：
--
作者：
J. V. D. Beucken;X. Walboomers;M. M. Vos;Najm Nico Sommerdijk;Rjm Roeland Nolte;Rjm Roeland Nolte;J. Jansen
由于DNA的结构特性，含DNA的生物材料涂层为软组织和硬组织植入物提供了潜在的有益效果。在目前的研究中，目的是评估在体外细胞和体内组织相容性的多层DNA涂层产生的静电自组装技术，与聚-D-赖氨酸或聚（烯丙胺盐酸盐）作为阴离子DNA的阳离子对应物。在钛基材上制备多层DNA涂层。未涂覆的钛基材作为对照。使用活/死测定和基于MTT的测定对大鼠原代真皮成纤维细胞（RDF）进行体外实验，评估其活力。多层DNA涂层的存在并不影响RDF细胞的活力。另一方面，在两种类型的多层DNA涂层上都证明了增殖增加。使用体内大鼠模型研究皮下植入植入物在植入4周和12周期间的软组织相容性。光学显微镜分析显示，所有植入物均被含有α-平滑肌肌动蛋白的纤维囊包围，并且多层DNA涂层的存在不会引起炎症和伤口愈合方面的任何不良反应。从组织形态学角度来看，根据多层DNA涂层或植入时间，未观察到囊膜质量或厚度的显著差异。本研究中证明的多层DNA涂层的细胞和组织相容性允许其使用和功能化与适当的化合物，以调节细胞和组织的反应，在牙科和医疗种植。
DNA-containing biomaterial coatings offer potential beneficial effects for both soft and hard tissue implants because of the structural properties of DNA. In the current study, the aim was to assess the in vitro cyto- and in vivo histocompatibility of multilayered DNA-coatings generated using the electrostatic self-assembly technique, with poly-D-lysine or poly(allylamine hydrochloride) as the cationic counterparts of anionic DNA. Multilayered DNA-coatings were fabricated on titanium substrates. Noncoated titanium substrates served as controls. In vitro experiments with rat primary dermal fibroblasts (RDF) assessing their viability were performed using a Live/Dead assay and an MTT-based assay. The presence of multilayered DNA-coatings did not affect RDF cell viability. On the other hand, an increased proliferation was demonstrated on both types of multilayered DNA-coatings. An in vivo rat model was used to study the soft tissue histocompatibility of subcutaneously inserted implants during implantation periods of 4 and 12 weeks. Light microscopic analysis revealed that all implants were surrounded by a fibrous capsule containing alpha-smooth muscle actin, and that the presence of a multilayered DNA-coating did not induce any adverse effects in terms of inflammation and wound healing. Histomorphometrically, no significant differences in capsule quality or thickness were observed dependent on multilayered DNA-coating or implantation period. The cyto- and histocompatibility of multilayered DNA-coatings demonstrated in this study allows their use and functionalization with appropriate compounds to modulate cell and tissue responses in dental and medical implantology.
影响因子：
14
作者：
Thapa, A;Miller, DC;Haberstroh, KM
通讯作者：
Haberstroh, KM