Intrarenal localization of the plasma membrane ATP channel pannexin1
Intrarenal localization of the plasma membrane ATP channel pannexin1
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DOI：
10.1152/ajprenal.00206.2011
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发表时间：
2012-11-01
影响因子：
4.2
通讯作者：
Peti-Peterdi, Janos
中科院分区：
文献类型：
--
作者：
Hanner, Fiona;Lam, Lisa;Peti-Peterdi, Janos
张建军，张建军，张建军，等。ATP通道pannexin1在肾内定位的研究进展。[J] .中华医学杂志，2012,31(3):559 - 559。2012年9月5日首次发表；doi: 10.1152 / ajprenal.00206.2011。在肾小管中，上皮细胞释放的ATP刺激嘌呤能受体，调节盐和水的重吸收。然而，ATP释放到管状管腔的机制是多方面的。Pannexin1 （Panx1）是一个新发现的基因。在质膜上形成连接蛋白样通道的无所不在的表达蛋白，已被证明是一种机械敏感的ATP通道。在这里，我们报道了Panx1在小鼠肾脏中的定位。免疫荧光法观察到沿肾小管顶端细胞膜的近端小管、细降肢和集合管中有强Panx1表达。在肾脉管系统中，Panx1的表达局限于肾动脉的血管平滑肌细胞，包括传入和传出小动脉。此外，我们通过测量野生型和Panx1（-/-）小鼠新鲜尿液样本的ATP含量来检测肾上皮细胞中表达的Panx1通道是否促进腔内ATP释放。与野生型小鼠相比，Panx1（-/-）组的尿ATP水平降低了30%。这些结果表明肾脏中的Panx1通道可能调节ATP的释放，并通过嘌呤能信号参与控制肾上皮液和电解质的运输和血管功能。
Hanner F, Lam L, Nguyen MT, Yu A, Peti-Peterdi J. Intrarenal localization of the plasma membrane ATP channel pannexin1. Am J Physiol Renal Physiol 303: F1454-F1459, 2012. First published September 5, 2012; doi:10.1152/ajprenal.00206.2011.-In the renal tubules, ATP released from epithelial cells stimulates purinergic receptors, regulating salt and water reabsorption. However, the mechanisms by which ATP is released into the tubular lumen are multifaceted. Pannexin1 (Panx1) is a newly identified. ubiquitously expressed protein that forms connexin-like channels in the plasma membrane, which have been demonstrated to function as a mechano-sensitive ATP conduit. Here, we report on the localization of Panx1 in the mouse kidney. Using immunofluorescence, strong Panx1 expression was observed in renal tubules, including proximal tubules, thin descending limbs, and collecting ducts, along their apical cell membranes. In the renal vasculature, Panx1 expression was localized to vascular smooth muscle cells in renal arteries, including the afferent and efferent arterioles. Additionally, we tested whether Panx1 channels expressed in renal epithelial cells facilitate luminal ATP release by measuring the ATP content of urine samples freshly collected from wild-type and Panx1(-/-) mice. Urinary ATP levels were reduced by 30% in Panx1(-/-) compared with wild-type mice. These results suggest that Panx1 channels in the kidney may regulate ATP release and via purinergic signaling may participate in the control of renal epithelial fluid and electrolyte transport and vascular functions.