Direct three-dimensional morphometric analysis of human cancellous bone:: Microstructural data from spine, femur, iliac crest, and calcaneus
Direct three-dimensional morphometric analysis of human cancellous bone:: Microstructural data from spine, femur, iliac crest, and calcaneus
复制标题
DOI：
10.1359/jbmr.1999.14.7.1167
复制
发表时间：
1999-07-01
影响因子：
6.2
通讯作者：
Rüegsegger, P
中科院分区：
文献类型：
--
作者：
Hildebrand, T;Laib, A;Rüegsegger, P
不同的骨骼部位和不同的疾病状态，松质骨结构的外观是不同的。在老化和疾病期间，钢板被打孔，连杆被溶解。从一种结构类型到另一种结构类型的转换是持续的。因此，传统的基于固定模型类型的组织形态计量学方法将导致可疑的结果。在骨研究中引入三维(3D)测量技术，使得在不假设结构类型的情况下捕捉松质骨的实际结构成为可能。然而，这需要直接利用3D信息的新方法。在欧盟BioMed I项目的框架内，我们分析了来自52个捐赠者的5个不同骨骼部位(股骨头、L2和L4椎体、髂骨和跟骨)的260例人骨活检标本。用微型计算机断层扫描仪对样本进行三维测量，然后用传统的间接组织形态计量学方法和新开发的直接组织形态计量学方法进行评估。结果表明，不同方法之间及其与骨体积分数的关系有显著差异。根据对人骨活检的直接3D分析，似乎骨量较低的样本的主要特征是板棒比较小，其次是骨小梁元素较薄。
The appearance of cancellous bone architecture is different for various skeletal sites and various disease states. During aging and disease, plates are perforated and connecting rods are dissolved. There is a continuous shift from one structural type to the other. So traditional histomorphometric procedures, which are based on a fixed model type, will lead to questionable results. The introduction of three-dimensional (3D) measuring techniques in bone research makes it possible to capture the actual architecture of cancellous bone without assumptions of the structure type. This requires, however, new methods that make direct use of the 3D information. Within the framework of a BIOMED I project of the European Union, we analyzed a total of 260 human bone biopsies taken from five different skeletal sites (femoral head, vertebral bodies L2 and L4, iliac crest, and calcaneus) from 52 donors. The samples were measured three-dimensionally with a microcomputed tomography scanner and subsequently evaluated with both traditional indirect histomorphometric methods and newly developed direct ones. The results show significant differences between the methods and in their relation to the bone volume fraction. Based on the direct 3D analysis of human bone biopsies, it appears that samples with a lower bone mass are primarily characterized by a smaller plate-to-rod ratio, and to a lesser extent by thinner trabecular elements.