Direct competitive ELISA enhanced by dynamic light scattering for the ultrasensitive detection of aflatoxin B-1 in corn samples
Direct competitive ELISA enhanced by dynamic light scattering for the ultrasensitive detection of aflatoxin B-1 in corn samples
复制标题
动态光散射增强直接竞争 ELISA，用于玉米样品中黄曲霉毒素 B-1 的超灵敏检测
DOI：
10.1016/j.foodchem.2020.128327
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发表时间：
2021
期刊：
影响因子：
8.8
通讯作者：
Xiong Yonghua
中科院分区：
文献类型：
--
作者：
Zhan Shengnan;Hu Jiaqi;Li Yu;Huang Xiaolin;Xiong Yonghua
Compared with absorbance, scattering-based dynamic light scattering (DLS) signal has higher sensitivity because its light-scattering intensity is very sensitive to changes in size, thereby enhancing the sensitivity. Herein, we first developed a DLS-enhanced direct competitive enzyme-linked immunosorbent assay (DLS-dcELISA) for ultrasensitive detection of aflatoxin B1(AFB1) in corn. By using hydroxyl radical-induced gold nanoparticle (AuNP) aggregation to amplify AuNP scattering signals, the developed DLS-dcELISA exhibited ultrahigh sensitivity for AFB1. The detection limit was 0.12 pg mL−1, which was 153- and 385-fold lower than those obtained using plasmonic and colorimetric dcELISA. In addition, the DLS-dcELISA exhibited excellent selectivity, high accuracy, and strong practicality. Overall, this work presented a simple and universal strategy for improving the sensitivity of traditional ELISA platform only by using the sensitive DLS signals. This technique can replace absorbance-based plasmonic or colored signals as immunoassay signal output for enhanced competitive detection of mycotoxins.
