Conformational toggle triggers a modulator of RNA polymerase activity
Conformational toggle triggers a modulator of RNA polymerase activity
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DOI：
10.1016/j.tibs.2006.06.004
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发表时间：
2006-08-01
影响因子：
13.8
通讯作者：
Hochschild, Ann
中科院分区：
文献类型：
--
作者：
Deighan, Padraig;Hochschild, Ann
最近发现的一类转录因子的成员，包括刺激转录物切割的Gre因子，通过直接调节RNA聚合酶（RNAP）的催化性质来发挥作用。现在，三个研究小组已经确定了Gre同系物Gfh 1的晶体结构，它抑制所有RNAP催化活性。引人注目的是，这些结构揭示了Gfhl和GreA构象之间令人困惑的差异，但pH依赖性构象切换改变Gfh1活性的发现表明了一个优雅的解决方案。
Members of a recently discovered class of transcription factor, which includes the Gre factors that stimulate transcript cleavage, function by directly modulating the catalytic properties of RNA polymerase (RNAP). Now, three research groups have determined crystal structures of a Gre homollog, Gfh1, which inhibits all RNAP catalytic activities. Strikingly, these structures reveal a puzzling discrepancy between the Gfhl and GreA conformations, but the discovery that a pH-dependent conformational toggle alters Gfh1 activity suggests an elegant solution.