Isolation of bacteriophages specific for the K1 polysaccharide antigen of Escherichia coli
Isolation of bacteriophages specific for the K1 polysaccharide antigen of Escherichia coli
复制标题
大肠杆菌 K1 多糖抗原特异性噬菌体的分离
DOI：
10.1128/jcm.6.6.548-550.1977
复制
发表时间：
1977
影响因子：
9.4
通讯作者：
B. Rowe
中科院分区：
文献类型：
--
作者：
R. Gross;T. Cheasty;B. Rowe
用大肠杆菌02:K1:H4（菌株U9/41）对污水样品进行富集后，制备了5个噬菌体库。通过抗血清-琼脂扩散技术，使用已知可检测大肠杆菌K1抗原的B群脑膜炎球菌抗血清，对噬菌体裂解224株大肠杆菌的能力进行了测试。以大肠杆菌K抗原2 ~ 99标准试验菌株为对照菌株。在使用抗血清-琼脂技术发现的101株具有K1抗原的菌株中，93株被至少一种噬菌体裂解，而123株明显缺乏K1的菌株中有8株被一种或多种噬菌体裂解。K抗原2至99的标准试验株均未被任何噬菌体裂解。8株被认为缺乏K1但被噬菌体裂解的菌株用B群脑膜炎球菌抗血清重新进行免疫电泳检测；8株菌株中有5株得到K1对应的沉淀线。K1特异性噬菌体的使用为鉴定K1抗原提供了一种廉价和简便的方法。
Five bacteriophage stocks were prepared after enrichment of a sewage sample using Escherichia coli 02:K1:H4 (strain U9/41). The bacteriophages were tested for their ability to lyse 224 strains of E. coli that had been tested for the presence of the K1 antigen by means of an antiserum-agar diffusion technique, using a meningococcus group B antiserum known to detect the E. coli K1 antigen. The standard test strains for E. coli K antigens 2 to 99 were used as control strains. Of the 101 strains found to possess the K1 antigen using the antiserum-agar technique, 93 were lysed by at least one of the bacteriophages, whereas 8 of the 123 strains apparently lacking K1 were lysed by one or more of the bacteriophages. None of the standard test strains for K antigens 2 to 99 was lysed by any of the bacteriophages. The eight strains thought to lack K1 but that were lysed by bacteriophage were re-examined by immunoelectrophoresis, using meningococcus group B antiserum; five of the eight strains gave a precipitin line corresponding to K1. The use of K1-specific bacteriophages offers an inexpensive and easy method for the identification of the K1 antigen.