Effect of neferine on toxicodynamics of dichlorvos for inhibiting rabbit cholinesterase.
Effect of neferine on toxicodynamics of dichlorvos for inhibiting rabbit cholinesterase.
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发表时间：
2003-04
影响因子：
8.2
通讯作者：
Y. Xiong;F. Zeng
中科院分区：
文献类型：
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作者：
Y. Xiong;F. Zeng
目的研究甲基莲心碱(Nef)对敌敌畏(DDVP)抑制家兔胆碱酯酶(ChE)毒效的影响，并探讨Nef体内外再激活作用的相关性。方法采用量热法测定敌敌畏血浓度，采用3P97软件计算毒代动力学参数。 Ellman 法用于测定血浆中 ChE 活性。绘制体内毒效曲线和体外浓度效应曲线，比较Nef对这两条曲线的影响。结果 家兔口服 12.5 mg/kg DDVP 中毒后，Nef 对 DDVP 的毒代动力学几乎没有影响。 Nef 7.5 mg/kg和氯吡毒肟(2-PAM Cl) 50 mg/kg治疗后，ChE降低的毒效曲线斜率增加约3倍，与未治疗组相比，差异有较高的显着性(P<0。05)。结论 Nef 重新激活了 DDVP 抑制的 ChE。体外实验可作为观察抗胆碱酯酶抑制的ChE重新激活的初步指标。
AIM To study the effect of neferine (Nef) on toxicodynamics of dichlorvos (DDVP) for inhibiting the rabbit cholinesterase (ChE) and search the relativity between in vivo and in vitro reactivating effect of Nef. METHODS Calorimetric method was used to determine the blood concentraiton of DDVP, and 3P97 software was used to calculate the parameters of toxicokinetics. Ellman's method was used to determine the ChE activity in plasma. The toxicodynamics curve in vivo and the concentration-effect curve in vitro were drawn to compare the effects of Nef on these two curves. RESULTS In the rabbit poisoned by ig DDVP 12.5 mg/kg there was almost no effect of Nef on the toxicokinetics of DDVP. The slope of toxicodynamics curves for depressed ChE increased about 3 times after treatment with Nef 7.5 mg/kg and pyratoxime methylchloride (2-PAM Cl) 50 mg/kg, compared with the untreated group, there was a higher significant difference (P0.05). CONCLUSION Nef reactivates the ChE depressed by DDVP. The experiment in vitro may be used as a preliminary index to observe the reactivation of ChE inhibited by anticholinesterases.