Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation
Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation
复制标题
SEW2871 封装的多层微结构钛通过巨噬细胞募集和免疫调节改善骨整合
DOI：
10.1016/j.apmt.2020.100673
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发表时间：
2020-09-01
影响因子：
8.3
通讯作者：
Cai, Kaiyong
中科院分区：
文献类型：
--
作者：
He, Ye;Xu, Kun;Cai, Kaiyong
医用钛研究的主要任务是提高体内骨整合能力。除了间充质基质细胞（MSCs）-成骨细胞谱系外，巨噬细胞在钛种植体的骨整合过程中也发挥着不可替代的作用。巨噬细胞的及时募集和有效的免疫调节是重要的。本论文首先通过双酸蚀法制备了微结构钛，赋予钛以巨噬细胞募集能力和免疫调节作用。然后通过层层自组装技术在微结构钛表面构建了含有巨噬细胞募集剂SEW 2871的明胶-壳聚糖多层膜。根据我们的结果，当SEW 2871缓慢释放时，制备的样品有效地诱导巨噬细胞迁移。巨噬细胞的抗炎反应增强，促炎反应减轻，这归因于表面粗糙度和润湿性的改变。巨噬细胞的这种免疫调节作用进一步促进了MSCs在体外的成骨分化，并在体内植入6周后获得了更好的骨整合。因此，本研究表明，通过调节巨噬细胞募集和免疫调节，也可以实现钛材料的良好骨整合，这意味着钛植入物改善的潜在策略。(C)2020爱思唯尔有限公司保留所有权利。
The main task for medical-used titanium researches is to improve the in vivo osteointegration. In addition to mesenchymal stromal cells (MSCs)-osteoblasts lineage, macrophages also undertake irreplaceable roles in the osteointegration process of a titanium implant. The timely recruitment and efficient immunomodulation of macrophages is important. In this work, to firstly endow macrophages recruitment ability and immunomodulation effects to titanium, we fabricated a micro-structured titanium via a double acid etching method. Then a gelatin-chitosan multilayer contained SEW2871 (a macrophage recruitment agent) was constructed on the micro-structured titanium by a layer-by-layer (LBL) self-assembly technique. According to our results, the prepared samples effectively induced macrophages migration when SEW2871 was slowly released. The anti-inflammatory response of macrophages was enhanced and the pro-inflammatory response was alleviated, which were attributed to the changed surface roughness and wettability. This immunomodulation effects of macrophages further improved the osteogenic differentiation of MSCs in vitro and obtained a better osteointegration in vivo after 6 weeks of implantation. Therefore, this study demonstrated that a favorable osteointegration of titanium materials could also be achieved by regulating macrophages recruitment and immunomodulation, which implying a potential strategy for titanium implants amelioration. (C) 2020 Elsevier Ltd. All rights reserved.