Inflammation and oxidative stress markers by pentoxifylline treatment in rats with chronic renal failure and high sodium intake
Inflammation and oxidative stress markers by pentoxifylline treatment in rats with chronic renal failure and high sodium intake
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DOI：
10.1016/j.arcmed.2006.08.010
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发表时间：
2007-01-01
影响因子：
7.7
通讯作者：
Paniagua, Ramon
中科院分区：
文献类型：
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作者：
Gallardo, Juan M.;de Carmen Prado-Uribe, Maria;Paniagua, Ramon
背景炎症是慢性肾功能衰竭（CRF）患者死亡的危险因素。预防细胞外液体积膨胀和使用某些药物，如戊茶碱（PF），可以减少炎症和氧化应激。本研究旨在分析膳食钠和PF治疗对CRF大鼠炎症和氧化应激标志物水平的影响。采用5/6肾切除法建立慢性肾功能衰竭模型。不同组的CRF大鼠接受低钠（LNa，饮食中含0.01%氯化钠[NaCl]）、正常钠（NNa，饮食中含0.05% NaCl）或高钠饮食（HNa，如NNa+饮用水中含0.015% NaCl）。另一组接受HNa + PF治疗（饮用水中25 mg/ kg）60天。检测循环肌酐、肿瘤坏死因子α（TNF-α）、亚硝酸盐、巯基、丙二醛（MDA）和晚期氧化蛋白产物（AOPP）。高钠摄入量与较高的血清肌酐水平相关（中位数;四分位距），LNa，1.255; 0.715，NNa，1.305; 0.495，HNa，2.015; 1.115 mg/dL（p
Background. Inflammation is a risk factor for mortality in patients with chronic renal failure (CRF). Prevention of extracellular fluid volume expansion and the use of certain drugs such as pentoxifylline (PF) may reduce inflammation and oxidative stress. The aim of this study is to analyze the effect of dietary sodium and PF treatment on the levels of inflammation and oxidative stress markers in rats with CRF.Methods. CRF was induced in rats by 5/6 nephrectomy. Different groups of rats with CRF received low sodium (LNa, 0.01% sodium chloride [NaCl] in the diet), normal sodium (NNa, 0.05% NaCl in the diet), or high sodium diet (HNa, as in NNa plus 0.015% NaCl in the drinking water). An additional group received HNa plus PF treatment (25 mg/ kg in the drinking water) for 60 days. Circulating creatinine, tumor necrosis factor alpha (TNF-alpha), nitrites, thiols, malondialdehyde (MDA), and advanced oxidation protein products (AOPP) were measured.Results. Higher sodium intake was associated with higher serum creatinine levels (median; interquartile range), LNa, 1.255; 0.715, NNa, 1.305; 0.495, HNa, 2.015; 1.115 mg/dL (p