CD4+ and CD8+ T cells mediate adoptive immunity to aerosol infection of Mycobacterium bovis bacillus Calmette-Guerin
CD4+ and CD8+ T cells mediate adoptive immunity to aerosol infection of Mycobacterium bovis bacillus Calmette-Guerin
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DOI：
10.1086/315466
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发表时间：
2000-05-01
影响因子：
6.4
通讯作者：
Britton, WJ
中科院分区：
文献类型：
--
作者：
Feng, CG;Britton, WJ
重组酶激活基因缺陷的过继转移模型本研究建立了以卡介苗（BCG）为抗原的RAG-1（-/-）小鼠模型，以BCG为抗原，经免疫、未分级分离的T细胞或经荧光激活细胞分选仪分离的T细胞亚群，4周后检测感染器官内细菌载量。脾脏比肺脏更有效地表达了免疫原性。虽然CD 4（+）或未分级的T细胞保护肺和脾，但CD 8（+）T细胞仅在脾中赋予显著的保护作用，而在肺中没有。结果证实，除了CD 4（+），CD 8（+）T细胞也发挥作用，在防止细菌传播，这种转移模型可能是有用的解剖T细胞对分枝杆菌感染的反应。
An adoptive-transfer model using recombinase activation gene-deficient (RAG-1(-/-)) mice was developed to evaluate CD4(+) and CD8(+) T cell responses to infection with Mycobacterium bovis bacillus Calmette-Guerin (BCG), After receiving immune, unfractionated T cells or T cell subsets isolated by fluorescence-activated cell sorter, the RAG-1(-/-) mice were exposed to aerosol BCG, and the bacteria load in the infected organs was examined 4 weeks later. Adoptive immunity was expressed more effectively in the spleens than in the lungs. Although CD4(+) or unfractionated T cells protected both lungs and spleens, CD8(+) T cells conferred significant protection only in the spleens and not in the lungs. The results confirm that in addition to CD4(+), CD8(+) T cells also play a role in the prevention of bacterial dissemination, This transfer model may be useful for dissecting T cell responses to mycobacterial infection.