Sequential formation of covalently bonded hydrogel multilayers through surface initiated photopolymerization
Sequential formation of covalently bonded hydrogel multilayers through surface initiated photopolymerization
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DOI：
10.1016/j.biomaterials.2005.08.025
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发表时间：
2006-03-01
期刊：
影响因子：
14
通讯作者：
Pérez-Luna, VH
中科院分区：
文献类型：
--
作者：
Kizilel, S;Sawardecker, E;Pérez-Luna, VH
描述了一种连续形成水凝胶多层膜的新方法。第一层的形成是基于水凝胶前体油曙红衍生表面的表面引发的光聚合。为了连接后续层，必须能够用曙红官能化中间水凝胶层。在本工作中，这是通过将聚（乙二醇）氨基丙烯酸酯（NH 2-PEG-Acr）沿着其它水凝胶前体如聚（乙二醇）二丙烯酸酯（PEG-DA）油引入到e-C中间层来实现的。侧胺基允许中间层与曙红的官能化，用于新的水凝胶层的后续光聚合。该过程可以顺序重复以构建多层水凝胶膜。NH 2-PEG-Acr单体可以通过共轭加成反应将半胱胺偶联到PEG-DA上形成。多层形成的方法可以允许在每个水凝胶层内掺入特定的功能或组合物，使得可以形成多功能膜。它也可以通过适当的水凝胶化程序来实现，以形成3-D水凝胶结构。使用可见光（514 nm）而不是紫外光的温和的光聚合条件将使该技术对于组织工程、药物递送、生物材料、生物传感器开发和其中并入的元素对UV光暴露敏感的其他情况特别有吸引力。(c)2005爱思唯尔有限公司保留所有权利。
A novel method for the sequential formation of hydrogel multilayers is described. Formation of the first layer is based on surface initiated photopolymerization of hydrogel precursors oil eosin derivatized surfaces. In order to attach subsequent layers it is necessary to be able to functionalize intermediate hydrogel layers with eosin. In the present work, this is accomplished by introducing poly(ethylene glycol) amino acrylate (NH2-PEG-Acr) along with other hydrogel precursors such as poly(ethylene glycol) diacrylate (PEG-DA) oil the e C intermediate layers. The pendant amine groups allow functionalization of the intermediate layers with eosin for subsequent photopolymerization of new hydrogel layers. The process can be repeated sequentially to construct multilayered hydrogel membranes. The NH2-PEG-Acr monomer can be formed by coupling cysteamine to PEG-DA by a conjugate addition reaction. The approach to multilayer formation could allow the incorporation of specific functionalities or compositions within each hydrogel layer so that multifunctional membranes can be formed. It could also be implemented through proper photopatterning procedures, for the formation of 3-D hydrogel structures. The mild photopolymerization conditions employed using visible (514nm), rather than ultraviolet light would make this technique especially attractive for tissue engineering, drug delivery, biomaterials, biosensor development and other situations where the elements incorporated are sensitive to UV light exposure. (c) 2005 Elsevier Ltd. All rights reserved.