Serine-rich ultra high sulfur protein gene expression in murine hair and skin during the hair cycle.
Serine-rich ultra high sulfur protein gene expression in murine hair and skin during the hair cycle.
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毛发周期期间小鼠毛发和皮肤中富含丝氨酸的超高硫蛋白基因表达。
DOI：
10.1016/s0021-9258(17)45371-3
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
G. Vogeli
中科院分区：
文献类型：
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作者：
L. Wood;M. Mills;N. Hatzenbuhler;G. Vogeli
为了研究毛发分化的调节，分离了鼠基因组克隆gUHS-SER-M16，其含有富含丝氨酸的超高硫蛋白基因家族的两个成员。其中一个基因gUHS-SER-1编码230个氨基酸，其中40%为半胱氨酸，23%为丝氨酸;另一个基因gUHS-SER-2编码223个氨基酸，其中41%为半胱氨酸，21%为丝氨酸。这两个基因之间的相似性为73%，并且在它们的编码区内都有几个10个氨基酸的重复。在预期的启动子区域中，存在包括"TATA"盒的几个相似区域，两个基因都没有"CAT"盒。在3 '非翻译区，不存在相似性，因此将来自该区域的片段用作RNA斑点印迹和原位杂交的杂交探针。RNA斑点杂交显示，在毛发生长的活跃期mRNA水平升高，而在静止期mRNA水平较低。原位杂交表明，在毛发周期的活跃期，不仅在形成毛发的髓质和内根鞘中，而且在皮肤表皮的上层中发现了超高硫蛋白基因的mRNA。
To study the regulation of hair differentiation, a murine genomic clone, gUHS-SER-M16, was isolated that contained two members of the family of serine-rich ultra high sulfur protein genes. One of the genes, gUHS-SER-1, encodes 230 amino acids with 40% cysteine and 23% serine; the other gene, gUHS-SER-2, encodes 223 amino acids with 41% cysteine and 21% serine. The similarity between the two genes is 73%, and both have several 10-amino acid repeats within their coding regions. In the prospective promoter region, there are several regions of similarity including a “TATA” box, with neither gene having a “CAT” box. At the 3' untranslated region, there is no similarity, and thus a fragment from this region was used as a hybridization probe for RNA dot-blots and for in situ hybridizations. The RNA dot-blot showed elevated levels of mRNA during the active phases of hair growth and low levels during the resting phases. In situ hybridizations show that mRNA for the ultra high sulfur protein gene is found during the active phases of the hair cycle not only in the medulla and the inner root sheath of the forming hair but also in upper layers of the epidermis of skin.