Neurobehavioral toxicity related to the exposure of weaning mice to low-level mercury vapor and methylmercury and influence of aging
Neurobehavioral toxicity related to the exposure of weaning mice to low-level mercury vapor and methylmercury and influence of aging
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DOI：
10.2131/fts.3.185
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发表时间：
2016-06
期刊：
影响因子：
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通讯作者：
Minoru Yoshida;Jin-Yong Lee;Hana Shimizu-Furusawa;M. Satoh;C. Watanabe
中科院分区：
文献类型：
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作者：
Minoru Yoshida;Jin-Yong Lee;Hana Shimizu-Furusawa;M. Satoh;C. Watanabe
将雌性C57 BL小鼠暴露于低水平的汞蒸气（Hg 0，0.096 mg/m3），并在生长期给予含有5 ppm甲基汞（MeHg）的溶液，以检查其对出生后神经行为功能的影响。暴露期为3 - 7周龄时的4周。在10周龄时，进行三种行为测试：旷场（OPF）测试、被动回避反应（PA）测试和八臂放射状迷宫（RM）测试。为了评估老化的影响，在79周龄时进行了额外的行为测试。关于在10至14周龄时进行的三项行为测试的结果，Hg 0/MeHg/Hg 0 +MeHg组与对照组之间没有显著差异。此外，在汞暴露完成后79至83周龄进行的行为测试中，每个暴露组和对照组之间也没有显著差异。汞暴露结束后脑组织中汞浓度以Hg 0 +MeHg组最高，其次为MeHg组和Hg 0组。Hg 0 +MeHg和MeHg组的值≤ 3.0 μg/g。Hg 0组的值≤ 1.0 μg/g。1年后，Hg 0/MeHg组和对照组之间的脑汞浓度没有差异。然而，在汞+甲基汞组，它是显着高于对照组，这表明汞在大脑中的消失是延迟相比，暴露于汞或甲基汞单独。结果表明，生长期低浓度Hg ~（0+）甲基汞暴露对仔鼠的神经行为无影响。然而，汞在大脑中的消失被延迟相比，单独暴露于汞或甲基汞。
Female C57BL mice were exposed to a low level of mercury vapor (Hg0, 0.096 mg/m3) and was given the solution containing 5-ppm methylmercury (MeHg) during the growth period to examine the influence on the neurobehavioral function after birth. Exposure period was 4 weeks at 3 to 7 weeks of age. At 10 weeks of age, three behavioral tests were conducted; open field (OPF) test, passive avoidance response (PA) test, and eight-arm radial maze (RM) test. To evaluate the influence of aging, additional behavioral tests were performed at 79 weeks of age. With respect to the results of the three behavioral tests conducted at 10 to 14 weeks of age, there were no significant differences between the Hg0/MeHg/Hg0+MeHg and control groups. Furthermore, there were also no significant differences between each exposure and control group on behavioral tests performed at 79 to 83 weeks of age after the completion of mercury exposure. The concentration of mercury in the brain after the completion of exposure was the highest in the Hg0+MeHg group, followed by the MeHg and Hg0 groups. The values in the Hg0+MeHg and MeHg groups were ≤ 3.0 μg/g. The value in the Hg0 group was ≤ 1.0 μg/g. There were no differences in the brain concentration of mercury after 1 year between the Hg0/MeHg and control groups. However, in the Hg0+MeHg group, it was significantly higher than in the control group, suggesting that the disappearance of mercury in the brain is delayed in comparison with the exposure to Hg0 or MeHg alone. These results showed that there was no influence of low-level Hg0+MeHg exposure during the growth period on neurobehavioral manifestation. However, the disappearance of mercury in the brain was delayed in comparison with the exposure to Hg0 or MeHg alone.