Further insights into the phylogeny of peniculid ciliates (Ciliophora, Oligohymenophorea) based on multigene data.
Further insights into the phylogeny of peniculid ciliates (Ciliophora, Oligohymenophorea) based on multigene data.
复制标题
DOI：
10.1016/j.ympev.2020.107003
复制
发表时间：
2020-10
影响因子：
4.1
通讯作者：
Meng Sun;Yuan Li;Xinglong Cai;Yongqiang Liu;Ying Chen;Xuming Pan
中科院分区：
文献类型：
--
作者：
Meng Sun;Yuan Li;Xinglong Cai;Yongqiang Liu;Ying Chen;Xuming Pan
青霉菌包括一个大目纤毛原生生物在低膜蜂纲有许多未解决的进化关系。在此，我们报道了27个新序列，包括18S rRNA、ITS1-5.8S- ITS2 rRNA、28S rRNA和8种青霉菌的线粒体氧合基因。我们基于这些标记和四基因串联数据集（18S rRNA、ITS1-5.8S- ITS2 rRNA、28S rRNA和cox1基因）进行了系统发育分析。主要发现有：1)青霉亚纲和草履虫科为单系性，frontonia属为非单系性；2)传统上，尿芯虫被认为是一个家族，多基因分析支持尿芯虫的等级，并始终将其作为青霉属的姐妹目，与Lynn（2008）的分类一致，尿芯虫和青霉属构成青霉属亚纲；3)关于frontonia属的多基因系统发育的差异和与形态学数据的冲突需要扩展和修订物种诊断，我们建议考虑III群的frontonia(包括f。didieri F。ocularis F。anatolica F。pusillaandF。秀丽隐杆线虫)；4)草履虫科的多基因分析支持了先前建立的5个亚属。草履虫被归入绿草履虫亚属。chlorelligeruminto subgenusViridoparamecium。
Peniculids comprise a large order of ciliated protists in Class Oligohymenophorea having many unresolved evolutionary relationships. Herein, we report 27 new sequences, including 18S rRNA, ITS1-5.8S- ITS2 rRNA, 28S rRNA and the mitochondrialcox1genes of eight peniculids. We conducted phylogenetic analyses based on each these markers and on a four-gene concatenated data set (18S rRNA, ITS1-5.8S- ITS2 rRNA, 28S rRNA, andcox1gene). The main findings are: 1) subclass Peniculia and family Parameciidae are monophyletic, with genusFrontoniaremaining non-monophyletic; 2) Urocentrids have traditionally been regarded as a family, multi-gene analyses support the rank of Urocentrida and consistently recovers this order as sister to Peniculida, and Urocentrida and Peniculida comprise subclass Peniculia in agreement with Lynn’s (2008) classification; 3) discrepancies between multiple-gene phylogenies, and conflicts with morphologic data regarding genusFrontonianecessitate expansion and revision of species diagnoses and we propose consideration of Group III ofFrontonia(includingF. didieri,F. ocularis,F. anatolica,F. pusillaandF. elegans) asincertae sedisin Peniculida; 4) multi-gene analyses of Parameciidae support five previously established subgenera.Paramecium buetschliiis placed in subgenusChloroparamecium,andP. chlorelligeruminto subgenusViridoparamecium.