Neuroprotective effect of 20(S)-ginseno side Rg3 on cerebral ischemia in rats
Neuroprotective effect of 20(S)-ginseno side Rg3 on cerebral ischemia in rats
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DOI：
10.1016/j.neulet.2004.10.030
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发表时间：
2005-02-10
影响因子：
2.5
通讯作者：
Liu, K
中科院分区：
文献类型：
--
作者：
Tian, JW;Fu, FH;Liu, K
本研究旨在探讨20（S）-吲哚醌Rg（3）对大鼠局灶性脑缺血的保护作用。采用WKY大鼠大脑中动脉闭塞（MCAO）模型。采用行为学检查评价中枢神经系统损害。脑片经氯化三苯基四氮唑（TTC）染色，计算脑梗死面积。采用氢清除法测定局部脑血流量（rCBF），分光光度法测定脑组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性、丙二醛（MDA）和三磷酸腺苷（ATP）含量。此外，在脑线粒体中评估呼吸控制比（RCR =状态3/状态4）。结果表明，舌下静脉注射20（S）-Rg（3）10和5 mg kg（-1）对大鼠局灶性脑缺血损伤有明显的神经保护作用，与对照组相比，能显著降低神经功能缺损评分，缩小梗死面积，增加rCBF。同时，20（S）-β-Rg（3）能明显改善脑缺血后线粒体能量代谢，对抗脑缺血后SOD、GSH-Px活性的降低和MDA含量的升高。提示20（S）-β-Rg（3）可能通过减少脂质过氧化物、清除自由基、改善能量代谢等途径对脑缺血损伤具有神经保护作用。(c)2004爱思唯尔爱尔兰有限公司保留所有权利。
This study was conducted to investigate the neuroprotective effects of 20(S)-ginsenoside Rg(3) on focal cerebral ischemia in rats. Middle cerebral artery occlusion (MCAO) model in male Wistar-Kyoto (WKY) rats was employed. The behavioral tests were used to evaluate the damage to central nervous system. The infarct area of brain was assessed in the brain slices stained with 2,3,5-triphenyltetrazolium chloride (TTC). Hydrogen clearance techniques were used to monitor regional cerebral blood flow (rCBF), spectrophotometric assay methods were used to determine the activities of superoxide dismutase (SOD) and glutathione-peroxidase (GSH-Px), contents of malondialdehyde (MDA) and adenosine triphosphate (ATP) of the brain. Furthermore, the respiratory control ratio (RCR =State 3/State 4) was assessed in the brain mitochondria. The results showed that sublingual vein injection of 20(S)-ginsenoside Rg(3) at doses of 10 and 5 mg kg(-1), but not 2.5 mg kg(-1) exhibited significant neuroprotective effects on rats against focal cerebral ischemic injury by markedly decreasing neurological deficit scores, reducing the infarct area and enhancing the rCBF compared with the control group. At the same time, 20(S)-ginsenoside Rg(3) significantly improved mitochondrial energy metabolism, antagonized decreases in SOD and GSH-Px activities and increase in MDA level induced by cerebral ischemia. All these findings suggest that 20(S)-ginsenoside Rg(3) might provide neuroprotection against the cerebral ischemia-induced injury in rat brain through reducing lipid peroxides, scavenging free radicals and improving the energy metabolism. (c) 2004 Elsevier Ireland Ltd. All rights reserved.