Bacterial pathogens of rice in the Kingdom of Cambodia and description of a new pathogen causing a serious sheath rot disease
Bacterial pathogens of rice in the Kingdom of Cambodia and description of a new pathogen causing a serious sheath rot disease
复制标题
DOI：
10.1111/j.1365-3059.2010.02310.x
复制
发表时间：
2010-10-01
期刊：
影响因子：
2.7
通讯作者：
Stodart, B.
中科院分区：
文献类型：
--
作者：
Cother, E. J.;Noble, D. H.;Stodart, B.
2005年至2007年对柬埔寨水稻病害的研究表明，由燕麦酸食菌亚种（Acidovorax avenae subsp.）引起的病害广泛发生。燕麦、剑兰伯克霍尔德菌、洋葱伯克霍尔德菌和菠萝泛菌。这是柬埔寨首次报告这些病原体。此外，还分离出了一种假单胞菌，该假单胞菌会引起类似于鞘褐腐病（由褐褐色假单胞菌引起）的广泛疾病。研究的菌株对水稻品种 Sen Pidau 和 IR 66 具有致病性，产生与田间观察到的症状相似但稍微不那么严重的症状。基于16S rDNA基因序列比较分析，结合细胞壁脂肪酸分析和代谢谱，分离菌株归属于假单胞菌属。该新种与褐褐色假单胞菌和恶臭假单胞菌的区别在于它们不能代谢D-果糖、D-半乳糖、D-半乳糖酸内酯、D-半乳糖醛酸、D-葡萄糖胺酸、D-葡萄糖醛酸、对羟基苯乙酸、D-糖二酸和尿刊酸。主要脂肪酸为C-16:0、汇总特征3（C-16:1 omega 7c 和C-16:1 omega 6c）和汇总特征8（C-18:1 omega 7c），占总数的80%。部分 16S rRNA 基因序列（1460 bp）是相同的，除了 6 个菌株之间有两个核苷酸变化外。典型培养数据库内致病菌株的比对显示，与 Pseudomonas parafulva AJ 2129(T) 的相似性为 99.7%，与 Pseudomonas parafulva AJ 2129(T) 的相似性为 99.2%，与 P. fulva IAM 1592(T) 的相似性为 99.2%，与 P. plecoglossicidia FPC 951(T) 的相似性为 98.9%，与 P. fuscovaginae MAFF 的相似性为 98.1% 301177(T)。根据这项多相研究的数据，有人提出从水稻中分离出的未知菌株代表了假单胞菌属的一个新物种。
A study of rice diseases in Cambodia from 2005 to 2007 showed widespread occurrence of diseases caused by Acidovorax avenae subsp. avenae, Burkholderia gladioli, B. cepacia and Pantoea ananatis. This is the first report of these pathogens in Cambodia. Additionally, a pseudomonad causing a widespread disease similar to sheath brown rot (caused by Pseudomonas fuscovaginae) was isolated. The studied strains were pathogenic to rice cvs Sen Pidau and IR 66, producing similar, though slightly less severe, symptoms to those observed in the field. Based on comparative 16S rDNA gene sequence analysis, combined with cell wall fatty acid analysis and metabolic profiles, the isolated strains were allocated to the genus Pseudomonas. The novel species were differentiated from Pseudomonas fuscovaginae and P. putida by their inability to metabolize D-fructose, D-galactose, D-galactonic acid lactone, D-galacturonic acid, D-glucosaminic acid, D-glucuronic acid, p-hydroxy phenylacetic acid, D-saccharic acid and urocanic acid. The major fatty acids were C-16:0, summed feature 3 (C-16:1 omega 7c and C-16:1 omega 6c) and summed feature 8 (C-18:1 omega 7c), representing 80% of the total. Partial 16S rRNA gene sequences (1460 bp) were identical, except for two nucleotide changes amongst the six strains. Alignment of the causal strains within type-culture databases revealed similarities of 99.7% with Pseudomonas parafulva AJ 2129(T), 99.2% with P. fulva IAM 1592(T), 98.9% with P. plecoglossicidia FPC 951(T), and 98.1% with P. fuscovaginae MAFF 301177(T). On the basis of data from this polyphasic study, it is proposed that the unknown strains isolated from rice represent a novel species of the genus Pseudomonas.