Tilapia prolactin cells are thermosensitive osmoreceptors
Tilapia prolactin cells are thermosensitive osmoreceptors
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罗非鱼催乳素细胞是热敏渗透压感受器
DOI：
10.1152/ajpregu.00027.2022
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Seale, Andre P.
中科院分区：
文献类型：
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作者：
Woo, Daniel W.;Malintha, G. H.;Celino-Brady, Fritzie T.;Yamaguchi, Yoko;Breves, Jason P.;Seale, Andre P.
广盐性和广温性莫桑比克罗非鱼（Oreochromis mossambicus）的吻侧远侧部（RPD）内的催乳素（PRL）细胞通过释放两种不同的催乳素亚型（PRL 188和PRL 177）来迅速响应低渗刺激。在这里，我们描述了环境相关的温度变化如何影响mRNA水平的prl 188和prl 177和免疫反应性催乳素从RPDs和分散的PRL细胞的释放。当在等渗条件（330 mosmol/kgH 2 O）下应用时，温度升高6°C刺激PRL 188和PRL 177从RPD和灌注下分散的PRL细胞中释放。当暴露于相同的温度变化时，约50%分散的PRL细胞的体积逐渐增加约8%，水通道阻断剂氯化汞部分抑制了这种反应。在它们对升高的温度作出反应之后，PRL细胞仍然对低渗刺激（280 mosmol/kgH 2 O）作出反应。瞬时电位香草酸4，一个参与低渗诱导的PRL释放的Ca 2+通道的mRNA表达升高，分别在6和24 h时，在分散的PRL细胞和RPDs中响应于温度的升高; prl 188和prl 177 mRNA不受影响。我们的研究结果表明，热敏PRL释放介导的，至少部分地，通过细胞体积依赖性途径类似于如何实现可接受的PRL释放。
Prolactin (PRL) cells within therostral pars distalis(RPD) of euryhaline and eurythermal Mozambique tilapia,Oreochromis mossambicus, rapidly respond to a hyposmotic stimulus by releasing two distinct PRL isoforms, PRL188and PRL177. Here, we describe how environmentally relevant temperature changes affected mRNA levels ofprl188andprl177and the release of immunoreactive prolactins from RPDs and dispersed PRL cells. When applied under isosmotic conditions (330 mosmol/kgH2O), a 6°C rise in temperature stimulated the release of PRL188and PRL177from both RPDs and dispersed PRL cells under perifusion. When exposed to this same change in temperature, ∼50% of dispersed PRL cells gradually increased in volume by ∼8%, a response partially inhibited by the water channel blocker, mercuric chloride. Following their response to increased temperature, PRL cells remained responsive to a hyposmotic stimulus (280 mosmol/kgH2O). The mRNA expression oftransient potential vanilloid 4, a Ca2+-channel involved in hyposmotically induced PRL release, was elevated in response to a rise in temperature in dispersed PRL cells and RPDs at 6 and 24 h, respectively;prl188andprl177mRNAs were unaffected. Our findings indicate that thermosensitive PRL release is mediated, at least partially, through a cell-volume-dependent pathway similar to how osmoreceptive PRL release is achieved.