ISOLATION AND FUNCTIONAL CHARACTERIZATION OF HUMAN INTESTINAL MUCOSAL LYMPHOID-CELLS
ISOLATION AND FUNCTIONAL CHARACTERIZATION OF HUMAN INTESTINAL MUCOSAL LYMPHOID-CELLS
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DOI：
10.1172/jci108719
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发表时间：
1977-01-01
影响因子：
15.9
通讯作者：
BOOKMAN, MA
中科院分区：
文献类型：
--
作者：
BULL, DM;BOOKMAN, MA
人结肠粘膜淋巴样细胞的活细胞悬液是通过用二硫苏糖醇、EDTA的无钙和无镁盐溶液和纯化的胶原酶连续处理组织来制备的。与外周血相比，肠淋巴细胞群中骨髓来源的细胞数量更多，特别是带有膜IG[免疫球蛋白]A的细胞;与巨噬细胞自发结合;与绵羊红细胞快速形成玫瑰花结;体外合成IG增加。两个群体中的胸腺来源细胞总数相等。空细胞数量减少，在细胞轴承膜IgD和IgM，并在响应植物血凝素。肠群中的巨噬细胞/单核细胞的大小、粒度、运动性、持续的玻璃粘附和吞噬活性增加。与外周血淋巴样细胞相比，人肠淋巴样细胞似乎构成更成熟和/或活化的细胞群。制备方法应有助于炎症性肠病、胃肠癌和肠道分泌免疫系统的研究。
Viable suspensions of human colonic mucosal lymphoid cells were prepared by sequential treatment of tissue with dithiothreitol, EDTA in Ca,- and Mg-free salt solutions and purified collagenase. The intestinal lymphocyte population, in comparison with that of peripheral blood, had greater numbers of bone marrow-derived cells, particularly cells bearing membrane Ig[immunoglobulin]A; showed spontaneous association with macrophages; underwent rapid rosette formation with sheep erythrocytes; and demonstrated increased in vitro synthesis of Ig. Total thymus-derived cells were equal in the 2 populations. Decreases were found in null cell numbers, in cells bearing membrane IgD and IgM, and in responsiveness to phytohemagglutinin. Macrophage/monocytes in the intestinal population were increased in size, granularity, motility, sustained glass adherence and phagocytic activity. Human intestinal lymphoid cells appear to constitute a cell population that is more mature and/or activated in comparison with the lymphoid cells of peripheral blood. The method of preparation should lend itself to the study of inflammatory bowel disease, gastrointestinal cancer and the intestinal secretory immune system.