Potent inhibitors of anthrax lethal factor from green tea
Potent inhibitors of anthrax lethal factor from green tea
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DOI：
10.1038/sj.embor.7400118
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发表时间：
2004-04-01
期刊：
影响因子：
7.7
通讯作者：
Garbisa, S
中科院分区：
文献类型：
--
作者：
Dell'Aica, I;Donà, M;Garbisa, S
炭疽致死因子（LF）在炭疽的发展中起着重要作用。LF由细胞内的保护性抗原（PA）递送，在那里它在MAPK-激酶的N-末端上发挥其金属蛋白酶活性。PA + LF对培养的巨噬细胞具有细胞毒性，静脉注射时可杀死Fischer 344大鼠。我们在这里描述的特性，一些多酚包含在绿色茶作为强大的抑制剂LF金属蛋白水解活性，以及如何的主要儿茶素的绿色茶，（-）表没食子儿茶素-3-没食子酸酯，防止LF诱导的死亡的巨噬细胞和Fischer 344大鼠。
The anthrax lethal factor (LF) has a major role in the development of anthrax. LF is delivered by the protective antigen (PA) inside the cell, where it exerts its metalloprotease activity on the N-terminus of MAPK-kinases. PA + LF are cytotoxic to macrophages in culture and kill the Fischer 344 rat when injected intravenously. We describe here the properties of some polyphenols contained in green tea as powerful inhibitors of LF metalloproteolytic activity, and how the main catechin of green tea, (-)epigallocatechin-3-gallate, prevents the LF-induced death of macrophages and Fischer 344 rats.