High-density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool.
High-density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool.
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DOI：
10.1111/pbi.12485
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发表时间：
2016-05
影响因子：
13.8
通讯作者：
Edwards KJ
中科院分区：
文献类型：
--
作者：
Winfield MO;Allen AM;Burridge AJ;Barker GL;Benbow HR;Wilkinson PA;Coghill J;Waterfall C;Davassi A;Scopes G;Pirani A;Webster T;Brew F;Bloor C;King J;West C;Griffiths S;King I;Bentley AR;Edwards KJ
在小麦中，育种系之间缺乏遗传多样性已被认为是未来产量增加的重要障碍。属于面包小麦的二级和三级基因库的物种具有更高水平的遗传变异性，并且是扩大其遗传基础的基因的重要来源。来自祖先和相关物种的新基因的渐渗已被广泛用于改善六倍体小麦的农艺性状，但这种方法由于缺乏可用于追踪引入的染色体片段的标记而受到阻碍。在这里，我们描述了大量的单核苷酸多态性，可用于基因型六倍体小麦，并确定和跟踪从各种来源的基因渗入的鉴定。我们已经使用超高密度Axiom®基因分型阵列验证了这些标记，以表征一系列二倍体、四倍体和六倍体小麦种质和小麦近缘种。为了便于使用这些，标记和相关的序列和基因型信息都可以通过一个交互式网站获得。
In wheat, a lack of genetic diversity between breeding lines has been recognized as a significant block to future yield increases. Species belonging to bread wheat's secondary and tertiary gene pools harbour a much greater level of genetic variability, and are an important source of genes to broaden its genetic base. Introgression of novel genes from progenitors and related species has been widely employed to improve the agronomic characteristics of hexaploid wheat, but this approach has been hampered by a lack of markers that can be used to track introduced chromosome segments. Here, we describe the identification of a large number of single nucleotide polymorphisms that can be used to genotype hexaploid wheat and to identify and track introgressions from a variety of sources. We have validated these markers using an ultra‐high‐density Axiom® genotyping array to characterize a range of diploid, tetraploid and hexaploid wheat accessions and wheat relatives. To facilitate the use of these, both the markers and the associated sequence and genotype information have been made available through an interactive web site.