Influence of cultivation temperature on the ligninolytic activity of selected fungal strains
Influence of cultivation temperature on the ligninolytic activity of selected fungal strains
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发表时间：
2006
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通讯作者：
Iroon Polytechniou-
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作者：
Iroon Polytechniou-
-.许多报道表明，胞外过氧化物酶和漆酶氧化持久性有机污染物。在这项研究中，选择菌株的木质素分解活性测定在氮充足的液体培养基。为了确定温度对木质素降解活性的影响，选择白色腐真菌在不同温度（15、20、25和30 PPC）下培养。锰过氧化物酶和漆酶的活性进行了测定，但没有检测到木质素过氧化物酶的所有菌株。同种菌株间差异显著。最大的漆酶和锰过氧化物酶活性获得Pleenostreatus sp.3达到27.8和26 U/L后，11和13天的培养在30 PPC。关键词：木质素降解酶，白色腐菌，过氧化物酶，木质素过氧化物酶，锰过氧化物酶，漆酶。
-. Many reports have shown that extracellular peroxidases and laccases oxidize persistent organic pollutants. In this study, the ligninolitic activities of selected strains were determined in a nitrogen-sufficient liquid medium. In order to determine the effect of temperature on ligninolytic activities, selected white rot fungi were incubated at different temperatures (15, 20, 25 and 30PPC). The activities of manganese peroxidase and laccase were determined but no lignin peroxidase was detected for all strains. Significant differences were revealed among strains of the same species. The maximum laccase and manganese peroxidase activity was obtained by Pleurotus ostreatus sp.3 attaining 27.8 and 26 U/l after 11 and 13 days of cultivation at 30PPC. Key-Words: Ligninolytic enzymes, White rot fungi, Peroxidases, Lignin peroxidase, Manganese peroxidase, Laccase.