Spontaneous recovery of hindlimb movement in completely spinal cord transected mice: a comparison of assessment methods and conditions
Spontaneous recovery of hindlimb movement in completely spinal cord transected mice: a comparison of assessment methods and conditions
复制标题
DOI：
10.1038/sj.sc.3101970
复制
发表时间：
2007-05-01
期刊：
影响因子：
2.2
通讯作者：
Guertin, P. A.
中科院分区：
文献类型：
--
作者：
Ung, R. V.;Lapointe, N. P.;Guertin, P. A.
研究设计：比较不同运动评定量表对Th9/10脊髓横断(TX)小鼠的评价结果。目的：用不同的运动评定量表评估TX小鼠的自发性康复，寻找最灵敏的评估TX小鼠康复水平的方法，以及与性别和条件相关的差异。地点：拉瓦尔大学医学中心，神经科学单元和拉瓦尔大学，魁北克市，魁北克省，加拿大魁北克市解剖和生理学系。方法：包括Basso，Beattie and Bresna han(BBB)，Basso小鼠评分(BMS)，Antri，Oral and Barthe(AOB)，用运动功能评分(MFS)和平均综合评分(ACOS)评价TX雄性和雌性TX小鼠在野外和跑台条件下的自发活动恢复情况。结果：运动功能评分在TX后5周逐渐增加。其他方法在TX后前2-3周检测到进行性增加，但在第4周和第5周没有任何显著进展。总的来说，每种方法获得的得分在男性和女性之间或在开阔场地和跑台条件下没有显著差异。结论：这些结果进一步证实了TX动物在没有干预的情况下运动功能恢复的有限但显著的增加。虽然每种方法都可以检测到微小程度的恢复，但ACOS方法具有足够的区分性，可以在TX后5周内检测到进行性变化。总而言之，无论是在野外还是在跑步机条件下，ACOS评定量表都是评估TX小鼠后肢自发活动恢复情况的最具鉴别力的方法。
Study design: To compare results obtained with a variety of locomotor rating scales in Th9/10 spinal cord transected (Tx) mice.Objectives: To assess spontaneous recovery with a variety of rating scales to find the most sensitive methods for assessing recovery levels in Tx mice and differences associated with gender and condition.Setting: Laval University Medical Center, Neuroscience Unit & Laval University, Department of Anatomy and Physiology, Quebec City, Quebec, Canada.Methods: Scales including the Basso, Beattie and Bresnahan (BBB), the Basso Mouse Score (BMS), the Antri, Orsal and Barthe (AOB), the Motor Function Score (MFS) and the Averaged Combined Score (ACOS) were used to assess, in open-field and treadmill conditions, spontaneous locomotor recovery in male and female Tx mice.Results: The ACOS scale revealed a progressive increase of spontaneous recovery during 5-weeks post-Tx. The other methods detected a progressive increase for the first 2-3 weeks post-Tx without any significant progress in weeks 4 and 5. Generally, scores obtained with each method were nonsignificantly different between males and females or between open-field and treadmill conditions.Conclusion: These results further confirm the existence of a limited but significant increase of locomotor function recovery, occurring without intervention, in Tx animals. Although each method could detect small levels of recovery, the ACOS method was discriminative enough to detect progressive changes up to 5 weeks post-Tx. In conclusion, the ACOS rating scale was the most discriminative method for assessing the spontaneous return of hindlimb movements found in Tx mice, both in open-field and treadmill conditions.