Cloning and characterization of a novel human chemokine receptor
Cloning and characterization of a novel human chemokine receptor
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DOI：
10.1006/bbrc.1997.7981
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发表时间：
1998-02-04
影响因子：
3.1
通讯作者：
Li, Y
中科院分区：
文献类型：
--
作者：
Fan, P;Kyaw, H;Li, Y
本研究报告了人类基因HCR的鉴定，该基因编码一种新的人类趋化因子受体。HCR基因的部分序列首次在人中性粒细胞cDNA文库中发现。利用中性粒细胞文库中表达序列标签（EST）探针，分离出全长HCR cDNA。HCR cDNA的开放阅读框预测了一个具有7个跨膜结构域的345个氨基酸的蛋白质。HCR基因与人类MIP-1a受体具有良好的同源性，氨基酸同源性为43.1%，氨基酸相似度为64.4%，与其他人类趋化因子受体如MCP-3受体、MCP-5受体和MCP-1受体也具有相当的序列同源性。Northern blot分析表明，HCR基因在免疫组织如脾、胎肝、淋巴结和骨髓中大量表达。在人的肺和心脏中也发现了强烈的表达。染色体定位研究表明，HCR基因位于人类染色体Xq13带内。我们的研究结果表明，HCR基因是一种新的趋化因子受体。(C) 1998学术出版社。
The present study reports the identification of a human gene, HCR, which encodes a novel human chemokine receptor. The partial sequence of the HCR gene was first found in a human neutrophil cDNA Library. With the use of an expressed sequence tag (EST) probe from the neutrophil library, the full length HCR cDNA was isolated. The open reading frame of HCR cDNA predicts a protein of 345 amino acids with seven transmembrane domain topography. The HCR gene exhibits good homology to human MIP-1a receptor with 43.1% amino acid identity and 64.4% amino acid similarity and also shows considerable sequence homology to other human chemokine receptors such as the MCP-3 receptor, MCP-5 receptor, and MCP-1 receptor. Northern blot analysis suggests that HCR gene is expressed abundantly in immunal tissues such as spleen, fetal liver, lymph node, and bone marrow. Strong expression was also found in human lung and heart. A chromosome mapping study indicated that HCR gene is positioned within human chromosome band Xq13. Our result suggests that HCR gene is a novel putative chemokine receptor. (C) 1998 Academic Press.