Comparison of 2.5% Sodium Hypochlorite and 2% Chlorhexidine Gel on Oral Bacterial Lipopolysaccharide Reduction from Primarily Infected Root Canals
Comparison of 2.5% Sodium Hypochlorite and 2% Chlorhexidine Gel on Oral Bacterial Lipopolysaccharide Reduction from Primarily Infected Root Canals
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DOI：
10.1016/j.joen.2009.06.011
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发表时间：
2009-10-01
影响因子：
4.2
通讯作者：
Vianna, Morgana E.
中科院分区：
文献类型：
--
作者：
Gomes, Brenda P. F. A.;Martinho, Frederico C.;Vianna, Morgana E.
简介：本研究旨在比较2.5%次氯酸钠（NaOCl）和2%洗必泰（CHX）化学机械预备对牙髓坏死和根尖周炎患者口腔细菌脂多糖（LPS）的清除效果。方法：选择54个根管。在化学机械制备之前（s1）和之后（Q）收集样品。将患牙随机分为两组：GI组，2.5%NaOCl组（n = 27）和GII组，CHX组（n = 27）。采用鲎试剂法定量内毒素。结果：100%的研究样品中存在内毒素，中位值为272内毒素单位（EU）/mL（GI）和152.46 EU/mL（GII）。作为化学机械制备的结果，LPS含量降低至中值86 EU/mL（GI）和85 EU/mL（GII）。胃肠道内毒素减少百分比值较高（P < .05）。结论：2.5%NaOCl和2%CHX凝胶不能有效清除感染根管内的内毒素。（I Endod 2009;35：1350-1353）
Introduction: This clinical study was conducted to compare the efficacy of chemomechanical preparation with 2.5% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) gel on eliminating oral bacterial lipo-polysaccharide (LPS) in teeth with pulp necrosis and apical periodontitis. Methods: Fifty-four root canals were selected. Samples were collect before (s1) and after chemomechanical preparation (Q). Teeth were randomly divided into groups: GI, 2.5% NaOCl (n = 27) and GII, CHX gel (n = 27). Limulus amebocyte lysate assay was used to quantify endotoxins. Results: Endotoxin was present in 100% of the samples investigated, with a median value of 272 endotoxin units (EU)/mL (GI) and of 152.46 EU/mL (GII). As a result of chemomechanical preparation, LPS content was reduced to a median value of 86 EU/mL (GI) and 85 EU/mL (GII). Higher percentage value of endotoxin reduction was found in GI (P < .05). Conclusions: The 2.5% NaOCl and 2% CHX gel were not effective in eliminating endotoxin from the primarily infected root canals. (I Endod 2009;35:1350-1353)