A Photo-Stable Indocyanine Green and Rapamycin Co-Delivery System Based on Poly(Glutamic Acid)-Modified Manganese Phosphate for Synergetic Tumor Therapy
A Photo-Stable Indocyanine Green and Rapamycin Co-Delivery System Based on Poly(Glutamic Acid)-Modified Manganese Phosphate for Synergetic Tumor Therapy
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基于聚谷氨酸修饰磷酸锰的光稳定吲哚菁绿和雷帕霉素联合递送系统用于协同肿瘤治疗
DOI：
10.1002/cnma.201900572
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发表时间：
2019
期刊：
影响因子：
3.8
通讯作者：
Zhang Yun
中科院分区：
文献类型：
--
作者：
Hao Yongwei;Zheng Cuixia;Qiang Hong;Chen Hongli;Wang Fan;Zhang Jinjie;Zhang Hongling;Wang Lei;Zhang Zhenzhong;Zhang Yun
一些锰基纳米材料会猝灭光敏剂的荧光，从而强烈猝灭荧光发射，并抑制由于光诱导电荷从激发的光敏剂到纳米材料的转移而产生的活性氧（ROS）。在本研究中，为了克服这些缺点，采用无定形多孔磷酸锰（MnP）纳米颗粒来负载吲哚菁绿（ICG），观察到更宽的ICG吸光宽度而不是减弱的荧光轮廓，从而在808nm照射下具有更高的稳定性和光疗效率。此外，自噬抑制明显削弱了ICG介导的乳腺癌细胞光疗。在此基础上，将自噬促进剂雷帕霉素（RAPA）和ICG共同负载到MnP纳米颗粒中，并进一步用生物相容性聚谷氨酸进行修饰。正如预期的那样，该稳定的纳米平台在低 pH 值下释放了 80.0±4.1% 的药物，而在正常 pH 值下释放了 32.0±4.8% 的药物，表明具有清晰的 pH 响应释放曲线。通过该系统将光疗与自噬促进剂结合起来可以实现协同结果，如较小的相对肿瘤体积 1.8±0.4 所证明的那样。总的来说，这项工作提供了第一种光稳定的锰基纳米材料，没有荧光猝灭曲线，可实现多种理想的治疗性能。
Some manganese‐based nanomaterials quench the fluorescence of photosensitizers, which strongly quenches fluorescence emission and suppresses reactive oxygen species (ROS) production due to the photo‐induced charge transfer from the excited photosensitizer to nanomaterials. In this study, to overcome these disadvantages, amorphous porous manganese phosphate (MnP) nanoparticles are used for loading indocyanine green (ICG), and a broader ICG absorbance width instead of weakened fluorescence profile is observed, resulting in higher stability and phototherapy efficiency under 808 nm irradiation. Moreover, autophagy inhibition obviously weakens the ICG‐mediated phototherapy to breast cancer cells. On this foundation, an autophagy promoter rapamycin (RAPA) and ICG are co‐loaded into the MnP nanoparticles, and further decorated with biocompatible poly(glutamic acid). As expected, this stable nanoplatform released 80.0±4.1% agents at low pH compared to that 32.0±4.8% at normal pH, indicating a clear pH‐responsive release profile. Uniting phototherapy with autophagy promoter by this system is found to achieve synergistic outcomes as evidenced by the smaller relative tumor volume of 1.8±0.4. Overall, this work provides the first photo‐stable manganese‐based nanomaterial without fluorescence quenching profile for achieving multiple desirable therapeutic performances.