ELISA VERSUS ROUTINE TESTS IN THE DIAGNOSIS OF PATIENTS WITH SYSTEMIC AND NEUROBRUCELLOSIS
ELISA VERSUS ROUTINE TESTS IN THE DIAGNOSIS OF PATIENTS WITH SYSTEMIC AND NEUROBRUCELLOSIS
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DOI：
10.1111/j.1699-0463.1988.tb05286.x
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发表时间：
1988-02-01
期刊：
影响因子：
2.8
通讯作者：
SHAKIR, RA
中科院分区：
文献类型：
--
作者：
ARAJ, GF;LULU, AR;SHAKIR, RA
采用酶联免疫吸附试验（ELISA）检测不同阶段布鲁氏菌病患者血清、中枢神经系统（CNS）布鲁氏菌病患者血清和脑脊液（CSF）中布鲁氏菌特异性IgG、IgM和IgA的含量。结果与培养结果、微凝集（MA）、孟加拉玫瑰（RB）玻片凝集（RB）和梅利氏布鲁氏菌染色抗原（SA）进行比较。急性布鲁氏菌病患者（296例）血清中IgM（100%）、IgG（97%）和IgA (98%) ELISA阳性，亚急性布鲁氏菌病患者（44例）血清中IgG（100%）、IgM（86%）和IgA (100%) ELISA阳性。然而，在慢性布鲁氏菌病患者（40）中，IgG和IgA持续呈阳性（100%），而IgM仅在33%的血清中呈阳性。MA和RB结果相似，急性（98%）和亚急性（84%）患者比慢性（61%）布鲁氏菌病患者阳性。SA和培养的阳性结果明显降低。在CNS型布鲁氏菌病患者（45例）的脑脊液中，ELISA检测IgG、IgM和IgA的阳性率分别为100%、20%和85%，而培养阳性率为13%，MA阳性率为25%，RB阳性率为22%。无中枢神经系统受累的布鲁氏菌病（66例）、非布鲁氏菌脑膜炎（62例）和无脑膜炎（144例）患者的脑脊液标本ELISA检测结果为阴性。因此，具有IgG、IgM和IgA谱的ELISA是诊断布鲁氏菌病患者，特别是慢性或中枢神经系统感染患者的首选检测方法。
Sera from patients in different stages of brucellosis as well as sera and cerebrospinal fluid (CSF) from patients with central nervous system (CNS) brucellosis and controls, were tested by ELISA for Brucella-specific IgG, IgM and IgA. The results were compared with culture findings, micro-agglutination (MA), slide agglutination with Rose Bengal (RB), and Brucella melitensis stained antigens (SA). In sera of patients with acute brucellosis (296), ELISA was positive for IgM (100%), IgG (97%) and IgA (98%), and comparable results were found in sera of patients with subacute brucellosis (44): IgG (100%), IgM (86%) and IgA (100%). However, in patients with chronic brucellosis (40), IgG and IgA were consistently positive (100%) while IgM was only positive in 33% of their sera. The MA and RB showed similar results, being more positive in patients with acute (98%) and subacute (84%) than in chronic (61%) brucellosis. The SA and culture showed significantly lower positive results. In the CSF of patients with CNS brucellosis (45), ELISA was positive in 100%, 20% and 85% for IgG, IgM and IgA, respectively, compared to 13% positive by culture, 25% by MA and 22% by RB. ELISA was negative in the CSF specimens from patients with brucellosis without CNS involvement (66), or meningitis other than Brucella (62), and no meningitis (144). Thus, ELISA with its IgG, IgM and IgA profiles is the test of choice in the diagnosis of patients with brucellosis, especially those with chronic or CNS infection.