Mapping of QTLs underlying tolerance to alkali at germination and early seedling stages in rice.
Mapping of QTLs underlying tolerance to alkali at germination and early seedling stages in rice.
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DOI：
10.1016/s1875-2780(09)60006-7
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发表时间：
2008-01-01
期刊：
影响因子：
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通讯作者：
Qian, Q.
中科院分区：
文献类型：
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作者：
Cheng HaiTao, Cheng HaiTao;Jang Hua, Jang Hua;Qian, Q.
以籼稻TN1与粳稻CJ06杂交的120个双单倍体（DH）群体为材料，对萌发期和苗期在0.15% Na2CO3胁迫下碱害的10个性状相对值进行了定量性状位点（qtl）检测。相关分析表明，萌发期碱害率（ADG）与相对萌发能（RGE）、相对发芽率（RGR）和相对萌发指数（RGI）呈极显著正相关，苗期碱害率（ADS）与相对苗高（RSH）、相对根长（RRL）、相对根数（RRN）和相对苗干重（RDW）呈极显著正相关。ADG和ADS可作为水稻耐碱性的评价指标。利用qtlnetwork2.0软件，对14个可加性qtl和13个上位性qtl进行了定位。在第3染色体上，在rm251 ~ rm3280同一区域检测到2个主效qtl；在第7染色体上，在RM3286 ~ RM1279之间定位到3个主效qtl。上位性分析表明，1、2、7和12号染色体上的RM1-RM1195、RM1358-RM290、RM1279-RM505和RM1246-RM519等上位性位点在碱胁迫下具有重要作用，包含两个或多个上位性qtl，表明这些qtl通过不同的互作方式发挥作用。在碱胁迫下的关键基因座中，有一个可能是多效性的，也有一些是相互密切联系的。与其他研究发现的qtl相比，部分耐碱qtl可能与部分耐盐qtl位于同一区域。我们推测本文中的qtl可能包括两种类型，一种是抵抗离子（K+或Na+）胁迫的qtl，另一种是抵抗高pH胁迫的qtl，后者可能在耐碱方面起重要作用。不同水稻品种对碱胁迫的抗性qtl不同；我们可以通过标记辅助选择将它们传销，培育出适合盐碱地生长的品种。
A double haploid (DH) population including 120 lines derived from a cross between indica rice TN1 and japonica rice CJ06 was used to detect quantitative trait loci (QTLs) for 10-trait relative values of alkali damage at germination period and early seedling stage under 0.15% Na2CO3 stress. Correlation analyses showed that alkali damage rate in germination period (ADG) had significant positive correlation with relative germination energy (RGE), relative germination rate (RGR) and relative germination index (RGI), and alkali damage rate at early seedling stage (ADS) had significantly correlation with relative seedling height (RSH), relative root length (RRL), relative root number (RRN) and relative seedling dry weight (RDW). ADG and ADS can be used as evaluation indexes of alkaline tolerance in rice. By using QTLNetwork 2.0 software, 14 additive QTLs and 13 epistatic QTLs controlling the tolerance to alkali were mapped. Two main effect QTLs were detected in same region, RM251-RM3280, on chromosome 3, and three main effect QTLs were mapped between RM3286 and RM1279 on chromosome 7. Epistasis analysis showed that some epistatic loci, like RM1-RM1195, RM1358-RM290, RM1279-RM505, and RM1246-RM519 on chromosome 1, 2, 7, and 12, respectively, were important under alkali stress, included two or more epistatic QTLs, indicating that the QTLs displayed their effects through different ways of interaction. Among the key loci under alkali stress one may be pleiotropic or some closely linked with each other. Comparing QTLs with those found in other research, some alkali resistance QTLs were likely to be in the same region with some salt resistance QTLs. A presumption was made that QTLs in this article might include two types, one for resistance to ion (K+ or Na+) stress and other for resistance to high pH stress, the latter may play an important role for tolerance to alkali. Many rice varieties have different resistance QTLs for alkali stress; we can pyramid them through marker-assistant selection to breed varieties suitable to growth in saline-alkalized land.