Abnormal expression of chromosomal rabbit β-globin gene in Saccharomyces cerevisiae
Abnormal expression of chromosomal rabbit β-globin gene in Saccharomyces cerevisiae
复制标题
兔染色体β珠蛋白基因在酿酒酵母中的异常表达
作者：
J. Beggs;J. Berg;A. Ooyen;C. Weissmann
将兔染色体 β-珠蛋白 DNA 的 5.1 kilobase-pair 片段连接至 pJDB219，该质粒由 pMB9、2-μ 酵母质粒和酵母 leu-2 基因组成。用含有珠蛋白 DNA 的杂交体转化的酿酒酵母产生了 β-珠蛋白特异性 RNA。与成熟的β-珠蛋白mRNA相比，这些转录物从5'端缺少20-40个核苷酸，包含所有小内含子并延伸到大内含子的大约中部。因此，在酵母细胞中未检测到初级β-珠蛋白转录本的剪接。
A 5.1 kilobase-pair segment of rabbit chromosomal β-globin DNA was joined to pJDB219, a plasmid consisting of pMB9, the 2-µ, yeast plasmid and the yeast leu-2 gene. Saccharomyces cerevisiae transformed with the globin DNA-containing hybrid produced β-globin-specific RNA. As compared to mature β-globin mRNA, these transcripts lacked 20–40 nucleotides from the 5′ end, contained all of the small intron and extended to about the middle of the large intron. Thus, no splicing of the primary β-globin transcript could be detected in the yeast cells.