Biologically improved nanofibrous scaffolds for cardiac tissue engineering
Biologically improved nanofibrous scaffolds for cardiac tissue engineering
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DOI：
10.1016/j.msec.2014.08.018
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发表时间：
2014-11-01
影响因子：
7.9
通讯作者：
Ramakrishna, S.
中科院分区：
文献类型：
--
作者：
Bhaarathy, V.;Venugopal, J.;Ramakrishna, S.
通过静电纺丝技术开发的纳米纤维结构为干细胞（包括负责再生医学的干细胞）的锚定、迁移和分化提供了有吸引力的细胞外基质条件。最近，由两种或更多种聚合物共混物组成的生物复合纳米纤维被更整齐地静电纺丝，以根据其应用获得具有所需功能和机械性能的支架。本研究的重点是一个这样的尝试，使用共聚物聚（L-乳酸）-co-聚（ε-己内酯）（PLACL），丝素蛋白（SF）和芦荟（AV）用于制造生物复合纳米纤维支架的心脏组织工程。所制造的电纺PLACL、PLACL/SF和PLACL/SF/AV纳米纤维支架的SEM显微照片是具有互连孔的多孔、无珠、均匀的纳米纤维，并且所获得的纤维直径分别在459 +/-22 nm、202 +/-12 nm和188 +/-16 nm的范围内。在室温下获得的PLACL、PLACL/SF和PLACL/SF/AV电纺毡的弹性模量分别为14.1 +/-0.7、9.96 +/-2.5和7.0 +/-0.9MPa。PLACL/SF/AV纳米纤维与PLACL相比具有更理想的性能，可用作柔性细胞支撑支架，用于修复心肌梗死（MI）。PLACL/SF和PLACL/SF/AV纳米纤维的接触缠结为51 +/-12度，而单独的PLACL为133 +/-15度。到第6天，与PLACL相比，PLACL/SF/AV纳米纤维中的心脏细胞增殖增加了21%，到第9天进一步增加到42%。与所有其他纳米纤维支架相比，在第9天观察到心脏表达蛋白肌球蛋白和连接蛋白43的共聚焦分析更好。结果表明，所制备的PLACL/SF/AV纳米纤维支架具有良好的心肌组织工程梗死心肌再生的潜力。(C)2014爱思唯尔有限公司版权所有。
Nanofibrous structure developed by electrospinning technology provides attractive extracellular matrix conditions for the anchorage, migration and differentiation of stem cells, including those responsible for regenerative medicine. Recently, biocomposite nanofibers consisting of two or more polymeric blends are electrospun more tidily in order to obtain scaffolds with desired functional and mechanical properties depending on their applications. The study focuses on one such an attempt of using copolymer Poly(L-lactic acid)-co-poly (epsilon-caprolactone) (PLACL), silk fibroin (SF) and Aloe Vera (AV) for fabricating biocomposite nanofibrous scaffolds for cardiac tissue engineering. SEM micrographs of fabricated electrospun PLACL, PLACL/SF and PLACL/SF/AV nanofibrous scaffolds are porous, beadless, uniform nanofibers with interconnected pores and obtained fibre diameter in the range of 459 +/- 22 nm, 202 +/- 12 nm and 188 +/- 16 nm respectively. PLACL, PLACL/SF and PLACL/SF/AV electrospun mats obtained at room temperature with an elastic modulus of 14.1 +/- 0.7, 9.96 +/- 2.5 and 7.0 +/- 0.9 MPa respectively. PLACL/SF/AV nanofibers have more desirable properties to act as flexible cell supporting scaffolds compared to PLACL for the repair of myocardial infarction (MI). The PLACL/SF and PLACL/SF/AV nanofibers had a contactangle of 51 +/- 12 degrees compared to that of 133 +/- 15 degrees of PLACL alone. Cardiac cell proliferation was increased by 21% in PLACL/SF/AV nanofibers compared to PLACL by day 6 and further increased to 42% by day 9. Confocal analysis for cardiac expression proteins myosin and connexin 43 was observed better by day 9 compared to all other nanofibrous scaffolds. The results proved that the fabricated PLACL/SF/AV nanofibrous scaffolds have good potentiality for the regeneration of infarcted myocardium in cardiac tissue engineering. (C) 2014 Elsevier B.V. All rights reserved.