Conservation and alteration of chromosome territory arrangements in thyroid carcinoma cell nuclei
Conservation and alteration of chromosome territory arrangements in thyroid carcinoma cell nuclei
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DOI：
10.1089/thy.2006.0328
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发表时间：
2007-06-01
期刊：
影响因子：
6.6
通讯作者：
Katoh, Ryohei
中科院分区：
文献类型：
--
作者：
Murata, Shin-ichi;Nakazawa, Tadao;Katoh, Ryohei
染色体区域(CTs)是间期细胞中单个染色体占据的核内亚区。本研究采用多色荧光原位杂交技术，对4例正常甲状腺组织(NT)、4例腺瘤性甲状腺肿(AG)、6例乳头状癌(PC)和2例未分化癌(UC)的10号、18号和19号染色体的核内CT定位进行了研究。在NT和AGs中，CS10和CS18的径向定位分别超过60%和80%，而CS19的径向定位在超过80%的细胞核中央。在PC中，CS10和CS18的径向定位模式与NT相似。在PC中，CS19位于中央的核比在NT细胞中少(p<；0.01)。另一方面，具有DNA扩增细胞的UC显示出CS10、CS18和CS19放射状位置的位置异常。这些发现表明，CT定位的改变可能与DNA扩增有关，并从形态上解释了伴随着染色质异常特征的核异型性。
Chromosome territories (CTs) are intranuclear subregions occupied by individual chromosomes in an interphase cell. In this study, we investigated intranuclear CT positionings of chromosomes 10 (CS10), 18 (CS18), and 19 (CS19) in epithelial cells from four normal thyroid tissue (NT), four adenomatous goiters (AGs), six papillary carcinomas (PCs), and two undifferentiated carcinomas (UCs) using the multicolor fluorescence in situ hybridization method. In the NT and AGs, the radial positionings of CS10 and CS18 were detected at the periphery of nuclei in more than 60% and 80% of cells, respectively, whereas the radial positioning of CS19 was in the central region of the nuclei in more than 80% of cells. In the PCs, radial positioning pattern of CS10 and CS18 were similar to that in the NT. The nuclei with centrally located CS19 in PCs were less frequent than those in NT cells ( p < 0.01). On the other hand, UCs with cells having DNA amplification demonstrated the locational abnormalities of the CS10, CS18, and CS19 radial positions. These findings indicate that alteration of CT positioning could be related to DNA amplification and, morphologically, may explain the nuclear atypia that accompanies the abnormal chromatin feature.