Continuous superporous agarose beds in radial flow columns
Continuous superporous agarose beds in radial flow columns
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DOI：
10.1016/s0021-9673(01)01027-5
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发表时间：
2001-08-03
影响因子：
4.1
通讯作者：
Larsson, PO
中科院分区：
文献类型：
--
作者：
Gustavsson, PE;Larsson, PO
连续型超孔琼脂糖床是色谱、生物催化和电泳的新型载体材料。床层由一块琼脂糖凝胶组成，由携带流体的孔隙均匀地横切，这些孔隙很容易在10-100 μ m的范围内变化。在这项工作中，制备了大直径床（60 mm），并在专门设计的径向流塔中使用。通过排粒径色谱实验研究了床层的基本色谱性质。在亲和层析应用中，用Cibacron Blue 3GA衍生一个床层，用于从粗牛心脏提取物中纯化乳酸脱氢酶。在一个生物转化应用中，一个床被提供固定化β -半乳糖苷酶并用于生产无乳糖牛奶。(C) 2001 Elsevier Science B.V.版权所有
Continuous superporous agarose beds constitute a new support material for chromatography, biocatalysis and electrophoresis. The bed consists of a single piece of agarose gel, homogeneously transected by flow-carrying pores, which easily can be varied in the range of 10-100 mum. In this work, large diameter beds (60 mm) were prepared and used in specially designed radial flow columns. The basic chromatographic properties of the beds were investigated by size-exclusion chromatography experiments. In an affinity chromatography application one bed was derivatized with Cibacron Blue 3GA and used for the purification of lactate dehydrogenase from a crude bovine heart extract. In a biotransformation application one bed was provided with immobilized beta -galactosidase and used in the production of lactose-free milk. (C) 2001 Elsevier Science B.V. All rights reserved.