Dicer-like Proteins Regulate the Growth, Conidiation, and Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis.
Dicer-like Proteins Regulate the Growth, Conidiation, and Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis.
复制标题
切丁样蛋白调节巴西橡胶树胶孢炭疽菌的生长、分生孢子和致病性
DOI：
10.3389/fmicb.2017.02621
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发表时间：
2017
影响因子：
5.2
通讯作者：
He C
中科院分区：
文献类型：
--
作者：
Wang Q;An B;Hou X;Guo Y;Luo H;He C
橡胶树炭疽菌是引起橡胶树炭疽病的一种半营养真菌。DICER样蛋白(DCL)是产生小RNA的核心酶。在本研究中，构建了两个编码胶状芽孢杆菌基因的DICER样蛋白的敲除突变体，并对两个蛋白的功能进行了研究。结果表明，DCL在球孢霉的生长、分生和致病过程中起着重要的调控作用，DCL1和DCL2之间存在功能冗余。显微镜分析和DAB染色表明，ΔDcl1ΔDcl2双突变株致病性下降的主要原因是穿透宿主细胞的能力丧失，而不是生长速度下降。蛋白质组学分析表明，DCL蛋白影响调节胶孢霉多种生物学过程的功能蛋白的表达。这些数据有助于更好地理解DCL蛋白在调节球囊孢子虫的发育和发病中的功能。
Colletotrichum gloeosporioides from Hevea brasiliensis is the hemibiotrophic fungi which could cause anthracnose in rubber trees. Dicer like proteins (DCL) were the core enzymes for generation of small RNAs. In the present study, the knocking-out mutants of two dicer like proteins encoding genes of C. gloeosporioides were constructed; and functions of two proteins were investigated. The results showed that DCL play important roles in regulating the growth, conidiation and pathogenicity of C. gloeosporioides; and there is a functional redundancy between DCL1 and DCL2. Microscopy analysis and DAB staining revealed that loss of penetration ability into the host cells, instead of the decreased growth rate, was the main cause for the impaired pathogenicity of the ΔDcl1ΔDcl2 double mutant. Proteomics analysis suggested that DCL proteins affected the expression of functional proteins to regulating multiple biological processes of C. gloeosporioides. These data lead to a better understanding of the functions of DCL proteins in regulating the development and pathogenesis of C. gloeosporioides.