Genomic analysis of a novel Rhodococcus (Prescottella) equi isolate from a bovine host
Genomic analysis of a novel Rhodococcus (Prescottella) equi isolate from a bovine host
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从牛宿主中分离出的新型马红球菌（Prescottella）的基因组分析
DOI：
10.1007/s00203-019-01695-z
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发表时间：
2019
影响因子：
2.8
通讯作者：
V. Sangal
中科院分区：
文献类型：
--
作者：
M. Paterson;D. Ranasinghe;J. Blom;L. Dover;I. Sutcliffe;Bruno S. Lopes;V. Sangal
马红球菌（Prescottella）在马驹中引起肺炎样感染，具有高死亡率，并且还可以感染许多其他动物。equis也作为一种机会性人类病原体出现。在这项研究中，我们已经测序了一个novelR的基因组。equisolate，B 0269，分离自牛宿主粪便。与其他七个已克隆的R.等基因组，包括来自马或人的那些，揭示了一个泛基因组，包括6876个基因，其中核心基因组中有4141个基因。275个基因是牛分离物特有的，大多数编码未知功能的假设蛋白质。然而，这些基因包括四个拷贝的terA和五个拷贝的terD基因，它们可能参与对化学胁迫的反应。毒力特性研究equiare与携带致病岛的大质粒的存在有关，包括来自thevapmultigene家族的基因。对已知毒力相关质粒（pVAPA、pVAPB和pVAPN）的蛋白质序列进行BLAST搜索，发现牛分离株B 0269中两个重叠群上存在相似的质粒骨架;然而，未鉴定出主要毒力相关基因vapA、vapBorvapN的同源物。总之，本研究证实了R.等基因组是高度保守的，并报告了牛分离株B 0269中存在一种显然是新的质粒，需要进一步表征以了解其与毒力特性的潜在关系。
Rhodococcus(Prescottella)equicauses pneumonia-like infections in foals with high mortality rates and can also infect a number of other animals.R. equiis also emerging as an opportunistic human pathogen. In this study, we have sequenced the genome of a novelR. equiisolate, B0269, isolated from the faeces of a bovine host. Comparative genomic analyses with seven other publishedR. equigenomes, including those from equine or human sources, revealed a pangenome comprising of 6876 genes with 4141 genes in the core genome. Two hundred and 75 genes were specific to the bovine isolate, mostly encoding hypothetical proteins of unknown function. However, these genes include four copies ofterAand five copies ofterDgenes that may be involved in responding to chemical stress. Virulence characteristics inR. equiare associated with the presence of large plasmids carrying a pathogenicity island, including genes from thevapmultigene family. A BLAST search of the protein sequences from known virulence-associated plasmids (pVAPA, pVAPB and pVAPN) revealed a similar plasmid backbone on two contigs in bovine isolate B0269; however, no homologues of the main virulence-associated genes,vapA,vapBorvapN, were identified. In summary, this study confirms thatR. equigenomes are highly conserved and reports the presence of an apparently novel plasmid in the bovine isolate B0269 that needs further characterisation to understand its potential involvement in virulence properties.