Collagen and glycopolymer based hydrogel for potential corneal application
Collagen and glycopolymer based hydrogel for potential corneal application
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DOI：
10.1016/j.actbio.2009.07.027
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发表时间：
2010-01-01
影响因子：
9.7
通讯作者：
Griffith, May
中科院分区：
文献类型：
--
作者：
Deng, Chao;Li, Fengfu;Griffith, May
合成了6-甲基丙烯酰基-α-D-半乳糖胺(MG)，并用傅立叶变换红外光谱(FTIR)、核磁共振光谱(核磁共振)和单晶X射线衍射法对其结构进行了表征。以聚乙二醇二丙烯酸酯为交联剂，用1-乙基-3-(3-二甲氨基丙基)碳二亚胺和N-羟基琥珀酰亚胺化学交联I型胶原蛋白，制备了一系列互穿聚合物网络(IPN)水凝胶。傅立叶变换红外光谱和固体碳-13核磁共振仪证实了糖共聚物MG成功地包埋在胶原水凝胶中。IPN水凝胶的光学特性与人类角膜的光学特性相当。与对照胶原水凝胶相比，聚合物MG的加入提高了水凝胶的拉伸强度和弹性模量。生物降解结果表明，聚合物MG提高了复合水凝胶对胶原酶的稳定性。体外实验结果表明，IPN水凝胶支持人角膜上皮细胞的黏附和增殖，并在抑制细菌黏附方面优于人角膜。综上所述，IPN水凝胶可能是一种很有前途的角膜板层角膜移植材料。(C)2009年Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
6-Methacryloyl-alpha-D-galactopyranose (MG) was synthesized, and characterized by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectrometry, and single-crystal X-ray diffraction. A series of interpenetrating polymer network (IPN) hydrogels was fabricated by simultaneously photo-curing MG crosslinked by poly(ethylene glycol) diacrylate and chemically crosslinking type I collagen with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide. The successful incorporation of the glycopolymer, polymer MG, into collagen hydrogel was confirmed by FTIR and solid-state C-13 NMR. The optical characteristics of the IPN hydrogels are comparable to those of human corneas. The tensile strength and modulus of the hydrogels are enhanced by incorporation of polymer MG in comparison to that of the control collagen hydrogel. Biodegradation results indicated that polymer MG enhanced the stability of the composite hydrogels against collagenase. In vitro results demonstrated that the IPN hydrogel supported the adhesion and proliferation of human corneal epithelial cells and outperformed human cornea in blocking bacteria adhesion. Taken together, the IPN hydrogel might be a promising material for use in corneal lamellar keratoplasty. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.