Microbiologic changes in subgingival plaque before and during the early period of orthodontic treatment
Microbiologic changes in subgingival plaque before and during the early period of orthodontic treatment
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DOI：
10.2319/030311-156.1
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发表时间：
2012-03-01
影响因子：
3.4
通讯作者：
Song, Jae-Seok
中科院分区：
文献类型：
--
作者：
Kim, Sang-Ho;Choi, Dong-Soon;Song, Jae-Seok
目的：评估龈下菌群的变化之前和期间的水平和对齐正畸stage using the polymerase chain reaction（PCR）methods.Materials and Methods：30名正畸患者（17名女性和13名男性;年龄16.7 +/- 6.5岁）被纳入本研究。在四个不同的时间，从左上中切牙的远颊龈缝、左下中切牙的远颊龈缝、左上第一磨牙的近颊龈缝和左下第一磨牙的近颊龈缝中采集龈下微生物样品：分别于基线、矫治器放置前（T1）、矫治器放置后1周（T2）、3个月（T3）、6个月（T4）进行统计学分析。从样本中提取DNA，采用基于16 S rRNA的PCR检测方法，确定牙周病病原体为伴放线菌聚集杆菌、坦那氏菌、直弯杆菌、腐蚀性艾肯菌、牙龈卟啉单胞菌、中间普雷沃菌、变黑普雷沃菌和齿垢密螺旋体。正畸矫治器放置后，T形、直肌和变黑P的发生率显著增加。对于其他种属，频率趋于增加，但未观察到统计学显著差异。的频率的变化，代表微生物不存在于T1，但新开发的T2，T3，和T4，是在磨牙高于在切牙。结论：正畸矫治器的放置影响龈下的微生物组成，即使在早期的正畸治疗，增加牙周致病菌的患病率，特别是在磨牙区。（角度正交。2012;82：254-260）。
Objective: To evaluate changes in subgingival microbiota before and during the leveling and alignment orthodontic stage using the polymerase chain reaction (PCR) method.Materials and Methods: Thirty orthodontic patients (17 females and 13 males; aged 16.7 +/- 6.5 y) were included in this study. Subgingival microbial samples were taken from the disto-buccal gingival crevice of the left upper central incisors, the left lower central incisors, the mesio-buccal gingival crevice of the left upper first molars, and the left lower first molars, at four different times: at baseline, before placement of orthodontic appliances (T1), and 1 week (T2), 3 months (T3), and 6 months after placement of orthodontic appliances (T4). DNA was extracted from the samples, and the 16S rRNA-based PCR detection method was used to determine the prevalence of Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobactor rectus, Eikenella corrodens, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, and Treponema denticola, which are considered as putative periodontopathogens.Results: The frequency of T forsythia, C rectus, and P nigrescens significantly increased after placement of orthodontic appliances. For the other species, the frequency tended to increase but no statistically significant difference was noted. The frequency of the change, representing microorganisms not existing at T1 but newly developing at T2, T3, and T4, was higher at the molars than at the incisors.Conclusion: The placement of orthodontic appliances affects the subgingival microbial composition even during the early period of orthodontic treatment, increasing the prevalence of periodontopathogens, especially in the molar region. (Angle Orthod. 2012;82:254-260.)