Fluorine-19 NMR studies of glucosyl fluoride transport in human erythrocytes.
Fluorine-19 NMR studies of glucosyl fluoride transport in human erythrocytes.
复制标题
人类红细胞中葡萄糖基氟化物转运的氟 19 NMR 研究。
DOI：
10.1016/s0006-3495(95)80051-2
复制
发表时间：
1995
影响因子：
3.4
通讯作者：
S. Gabel
中科院分区：
文献类型：
--
作者：
R. London;S. Gabel
氟 19 磁化转移研究已用于测量平衡交换条件下吡喃葡萄糖基氟化物的传输速率。虽然可以测定 β-D-吡喃葡萄糖基氟化物的速率常数和渗透率，但发现 α-D-吡喃葡萄糖基氟化物的交换速率太慢，无法使用此方法进行测定。 β-吡喃葡萄糖基氟化物随时间的分解也限制了该物种数值结果的准确性；然而，很明显，α 和 β 形式的渗透率显着不同，即 P beta > P alpha。这一观察结果与最近对 n-氟-n-脱氧葡萄糖的观察结果相反，其中 n=2、3、4 或 6 时，P α > P β。这种差异可以用简单的交替构象模型来解释，其中一种构象（具有外部糖结合位点）表现出对分子的 β 形式的偏好，而第二种构象（具有内部糖结合位点）表现出对 α 形式的偏好。氟/羟基取代通过选择性地减少与其中一种构象的结合来揭示这些偏好，具体取决于氟化的具体位点。
Fluorine-19 magnetization transfer studies have been used to measure the transport rate of glucopyranosyl fluorides under equilibrium exchange conditions. Although rate constants and permeabilities could be determined for beta-D-glucopyranosyl fluoride, the exchange rate for alpha-D-glucopyranosyl fluoride was found to be too slow for determination using this method. The time-dependent decomposition of the beta-glucopyranosyl fluoride also limits the accuracy of the numerical results for this species; however, it is clear that the permeabilities of the alpha and beta forms differ significantly, i.e., P beta > P alpha. This observation is in contrast to recent observations for n-fluoro-n-deoxyglucose, for which P alpha > P beta for n=2, 3, 4, or 6. The difference can be explained in terms of a simple alternating conformation model in which one of the conformations (with an external sugar-binding site) exhibits a preference for the beta form of the molecule, while the second conformation (with an internal sugar binding site) exhibits a preference for the alpha form. Fluorine/hydroxyl substitutions unmask these preferences by selectively reducing the binding to one of the conformations, depending on the specific site of fluorination.
1-氟-D-吡喃葡萄糖基氟化物与葡萄糖苷酶的相互作用。
DOI：
10.1042/bj2790587
发表时间：
1991
期刊：
The Biochemical journal
影响因子：
--
作者：
Konstantinidis,A;Sinnott,ML
通讯作者：
Sinnott,ML