Raman spectroscopy reveals differences in collagen secondary structure which relate to the levels of mineralisation in bones that have evolved for different functions
Raman spectroscopy reveals differences in collagen secondary structure which relate to the levels of mineralisation in bones that have evolved for different functions
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DOI：
10.1002/jrs.4038
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发表时间：
2012-09-01
影响因子：
2.5
通讯作者：
Goodship, Allen E.
中科院分区：
文献类型：
--
作者：
Buckley, Kevin;Matousek, Pavel;Goodship, Allen E.
骨是一种复合材料，包括胶原纤维支架，周围是碳化羟基磷灰石矿物晶体。众所周知，成熟骨骼中矿物质和胶原蛋白的相对比例并不是确定的，而是为了“调整”其机械性能而进行调整的。然而，目前尚不清楚矿物质与胶原蛋白的比例是如何控制的。本文使用拉曼光谱（允许探测骨骼的矿物和胶原蛋白阶段）来探索控制机制与胶原蛋白性质有关的假设，并且不同矿化水平的骨骼具有不同质的胶原蛋白。展示了具有不同矿化水平的功能适应性骨骼的拉曼光谱，并强调了表明胶原二级结构（酰胺I带谱）和翻译后修饰（羟脯氨酸/脯氨酸比率）差异的特征。该研究表明，拉曼光谱可以提供一种手段来研究控制骨骼中矿物质与胶原蛋白比例的机制。了解这些机制可以为治疗性改变矿物质与胶原蛋白的比例铺平道路，从而控制骨骼的机械特性。版权所有：John Wiley & Sons， Ltd
Bone is a composite material comprising a collagen fibril scaffold surrounded by crystals of carbonated-hydroxyapatite mineral. It is well established that the relative proportions of mineral and collagen in mature bone are not definite and are adapted in order to 'tune' its mechanical properties. It is not known, however, how the mineral to collagen ratio is controlled. This paper uses Raman spectroscopy (which permits the probing of both the mineral and the collagen phases of bone) to explore the hypothesis that the control mechanism is related to the nature of the collagen and that bones with different levels of mineralisation have qualitatively different collagen. Raman spectra of functionally adapted bones with varying levels of mineralisation are presented and features that indicate the differences in the collagen's secondary structure (amide I band profiles) and post-translational modification (hydroxyproline/proline ratios) are highlighted. The study demonstrates that Raman spectroscopy can provide a means to investigate the mechanisms that control the mineral to collagen ratio of bone. Understanding these mechanisms could pave the way towards the therapeutic alteration of the mineral to collagen ratio and, thus, the control of the mechanical properties of bone. Copyright (C) 2012 John Wiley & Sons, Ltd.