Regulatory Role of a Receptor-Like Kinase in Specifying Anther Cell Identity
Regulatory Role of a Receptor-Like Kinase in Specifying Anther Cell Identity
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受体样激酶在指定花药细胞身份中的调节作用
DOI：
10.1104/pp.16.00016
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发表时间：
2016
期刊：
影响因子：
7.4
通讯作者：
Zhang Dabing
中科院分区：
文献类型：
--
作者：
Yang Li;Qian Xiaoling;Chen Mingjiao;Fei Qili;Meyers Blake C.;Liang Wanqi;Zhang Dabing
在开花植物中，花药细胞类型的顺序形成是一个高度有序的过程，对于成功的减数分裂和有性生殖至关重要。分生组织细胞的分化和细胞间通讯是协调花药发育的重要机制。细胞命运特化的拟议机制包括细胞表面定位的富亮氨酸重复序列受体样激酶（LRR-RLK）及其推定配体。在这里，我们提出的遗传和生化证据表明，水稻（Oryza sativa）LRR-RLK，MSP 1（多孢子细胞1），与其配体OsTDL 1A（TPD 1-like 1A）相互作用，指定花药壁层和小孢子母细胞的细胞身份。体外实验表明，OsTDL 1A的21个氨基酸的肽与MSP 1的LRR结构域具有物理相互作用。结果表明，突变体中缺少中间层和绒毡层细胞，小孢子母细胞数量增加，与突变体中的突变体相似，表明OsTDL 1A-MSP 1调控花药发育的途径相同。全基因组表达谱显示编码转录因子的基因表达改变，特别是碱性螺旋-环-螺旋和碱性亮氨酸拉链结构域转录因子inostdl 1aandmsp 1。在这些表达减少的基因中，一个puminase编码TGA（TGACGTCA顺式元件结合蛋白）因子OsTGA 10，另一个puminase编码植物特异性CC型谷氧还蛋白OsGrx_I1。OsTGA 10与OsGrx_I1相互作用，表明OsTDL 1A-MSP 1信号直接或间接影响花药细胞的氧化还原状态。总的来说，这些数据表明OsTDL 1A-MSP 1信号通路在指定水稻体细胞身份和抑制孢原细胞过度增殖中起着核心作用。
In flowering plants, sequential formation of anther cell types is a highly ordered process that is essential for successful meiosis and sexual reproduction. Differentiation of meristematic cells and cell-cell communication are proposed to coordinate anther development. Among the proposed mechanisms of cell fate specification are cell surface-localized Leu-rich repeat receptor-like kinases (LRR-RLKs) and their putative ligands. Here, we present the genetic and biochemical evidence that a rice (Oryza sativa) LRR-RLK, MSP1 (MULTIPLE SPOROCYTE1), interacts with its ligand OsTDL1A (TPD1-like 1A), specifying the cell identity of anther wall layers and microsporocytes. An in vitro assay indicates that the 21-amino acid peptide of OsTDL1A has a physical interaction with the LRR domain of MSP1. Theostdl1a msp1double mutant showed the defect in lacking middle layers and tapetal cells and having an increased number of microsporocytes similar to theostdl1aormsp1single mutant, indicating the same pathway of OsTDL1A-MSP1 in regulating anther development. Genome-wide expression profiles showed the altered expression of genes encoding transcription factors, particularly basic helix-loop-helix and basic leucine zipper domain transcription factors inostdl1aandmsp1. Among these reduced expressed genes, one putatively encodes a TGA (TGACGTCA cis-element-binding protein) factor OsTGA10, and another one encodes a plant-specific CC-type glutaredoxin OsGrx_I1. OsTGA10 was shown to interact with OsGrx_I1, suggesting that OsTDL1A-MSP1 signaling specifies anther cell fate directly or indirectly affecting redox status. Collectively, these data point to a central role of the OsTDL1A-MSP1 signaling pathway in specifying somatic cell identity and suppressing overproliferation of archesporial cells in rice.