The inflammatory response and hyaluronan synthases in the rabbit flexor tendon and tendon sheath following injury
The inflammatory response and hyaluronan synthases in the rabbit flexor tendon and tendon sheath following injury
复制标题
DOI：
10.1016/j.jhse.2007.05.017
复制
发表时间：
2007-10-01
影响因子：
1.8
通讯作者：
Wiig, M.
中科院分区：
文献类型：
--
作者：
Berglund, M.;Hart, D. A.;Wiig, M.
采用兔屈肌腱损伤模型，在损伤后3、6、12和24天用逆转录聚合酶链式反应检测参与炎症和纤维化过程的一组相关分子的mRNA水平。损伤后肌腱和腱鞘中COX-2、IL-1β、MMP-13和TIMP-I的表达均升高，且各分子的表达均呈组织和时间依赖性变化。MMP-13和TIMP-1mRNA在两种组织中的表达均显著上调，而COX-2和IL-1β在肌腱中的表达明显增加。透明质酸合成酶(HAS)2和3在损伤后肌腱组织中的mRNA水平均有升高，其中2的表达更明显。这些发现支持这样的概念，即屈肌腱和鞘的愈合涉及不同的分子事件，如果要加强愈合和减少粘连，每个组织可能需要独特的修改。
Using a rabbit model of flexor tendon injury, mRNA levels for a subset of relevant molecules involved in inflammatory and fibrotic processes were assessed by reverse transcriptase-polymerase chain reaction 3, 6, 12 and 24 days after injury. Increased levels of COX-2, IL-1 beta, MMP-13 and TIMP-I mRNA were detected in both tendon and tendon sheath following injury, with each molecule exhibiting tissue and time-dependent changes. MMP-13 and TIMP-1 mRNA levels were markedly upregulated in both tissues, whereas COX-2 and IL-1 beta predominantly increased in tendon. Both hyaluronan synthase (HAS) 2 and 3 exhibited increases in mRNA levels in tendon tissue after injury, HAS 2 being more pronounced. These findings support the concept that healing in the flexor tendon and the sheath involve different molecular events and that each tissue may require unique modifications if healing is to be enhanced and adhesions reduced.