Expression and distribution of Id helix-loop-helix proteins in human astrocytic tumors
Expression and distribution of Id helix-loop-helix proteins in human astrocytic tumors
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DOI：
10.1002/glia.10076
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发表时间：
2002-06-01
期刊：
影响因子：
6.2
通讯作者：
Aronica, E
中科院分区：
文献类型：
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作者：
Vandeputte, DAA;Troost, D;Aronica, E
Id家族的螺旋-环-螺旋蛋白参与多种过程，如发育、增殖和血管生成。在这项研究中，我们研究了Id 1，Id 2和Id 3在人类神经胶质肿瘤手术标本中的表达模式。Western blot分析表明，所有三个Id蛋白表达在星形细胞肿瘤。高级别肿瘤中的表达水平高于低级别肿瘤。免疫组织化学分析证实，许多肿瘤星形胶质细胞表现出强烈的Id 1 -3 IR。相反，在成人正常脑，Id表达低，在静息星形胶质细胞和内皮细胞。在肿瘤细胞中，Id蛋白显示胞质以及核定位。肿瘤细胞Id 1 -3 IR评分与增殖指数呈正相关。此外，Id 1 -3 IR在星形细胞肿瘤血管的内皮细胞中检测到。血管Id 1 -3的表达与肿瘤的血管分布和分级呈正相关。这些结果支持Id基因家族在增强肿瘤星形胶质细胞增殖潜力中的作用。证据还支持Id基因家族参与肿瘤血管生成，这是一个严重影响神经胶质肿瘤恶性行为的过程。
The Id family of helix-loop-helix proteins is involved in a variety of processes, such as development, proliferation, and angiogenesis. In this study, we investigated the expression pattern of Id1, Id2, and Id3 in surgical specimens of human glial tumors. Western blot analysis demonstrated that all three Id proteins were expressed in astrocytic tumors. Expression levels in high-grade tumors were higher than in low-grade tumors. Immunohistochemical analysis confirmed that many of the tumor astrocytes exhibited strong Id1-3 IR. In contrast, in adult human normal brain, Id expression was low both in resting astrocytes and in endothelial cells. In tumor cells, Id proteins displayed cytoplasmic as well as nuclear localization. Id1-3 IR scores in tumor cells were positively correlated with proliferation indices. Moreover, Id1-3 IR was detected in endothelial cells of the astrocytic tumor blood vessels. The vascular Id1-3 expression correlated positively with tumor vascularity and grade. These results support the role of the Id gene family in the enhanced proliferative potential of tumor astrocytes. The evidence also supports the involvement of the Id gene family in tumor angiogenesis, a process that critically influences the malignant behavior of glial tumors.