VILLIN, INTESTINAL BRUSH-BORDER HYDROLASES AND KERATIN POLYPEPTIDES IN INTESTINAL METAPLASIA AND GASTRIC-CANCER - AN IMMUNOHISTOLOGIC STUDY EMPHASIZING THE DIFFERENT DEGREES OF INTESTINAL AND GASTRIC DIFFERENTIATION IN SIGNET RING CELL CARCINOMAS
VILLIN, INTESTINAL BRUSH-BORDER HYDROLASES AND KERATIN POLYPEPTIDES IN INTESTINAL METAPLASIA AND GASTRIC-CANCER - AN IMMUNOHISTOLOGIC STUDY EMPHASIZING THE DIFFERENT DEGREES OF INTESTINAL AND GASTRIC DIFFERENTIATION IN SIGNET RING CELL CARCINOMAS
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DOI：
10.1007/bf00783022
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发表时间：
1988-01-01
期刊：
影响因子：
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通讯作者：
WEBER, K
中科院分区：
文献类型：
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作者：
OSBORN, M;MAZZOLENI, G;WEBER, K
胃癌已被检测绒毛蛋白的存在和小肠水解酶氨肽酶N和蔗糖酶异麦芽糖酶。这些蛋白质似乎不存在于正常的胃上皮细胞中。然而，胃的肠上皮化生和胃癌的腺型中的肿瘤细胞对所有三种标记物均呈阳性，显示特征性的顶端阳性。相反，在弥漫性胃癌中，这些标记物阳性的印戒细胞的百分比在10-100%之间变化，每种标记物显示出相似的阳性细胞百分比。用对不同角蛋白多肽特异的抗体检测胃癌显示，虽然所有7个肿瘤均为角蛋白8和18阳性，但2个也为角蛋白7阳性。在角蛋白7阳性肿瘤中，所有肿瘤细胞角蛋白7阳性。角蛋白8抗体也对常规固定的标本起反应。因此，胃癌揭示了不同程度的胃和肠分化
Gastric carcinomas have been assayed for the presence of villin and for the small intestinal hydrolases aminopeptidase N and sucrase isomaltase. These proteins seem not to be present in normal stomach epithelium. However intestinal metaplasia in stomach, and tumour cells in the glandular patterns of gastric carcinoma were positive for all three markers, showing characteristic apical positivity. In contrast, in diffuse gastric carcinomas the percentage of signet ring cells positive for these markers varied from 10–100% with each marker showing a similar percentage of positive cells. Testing of gastric carcinomas with antibodies specific for different keratin polypeptides showed that while all 7 tumours were positive for keratins 8 and 18,2 were also positive for keratin 7. In the keratin 7 positive tumours all tumour cells were keratin 7 positive. The keratin 8 antibody also reacted on routinely fixed specimens. Thus gastric carcinomas reveal different degrees of gastric and intestinal differentiation