In vitro vRNA-vRNA interactions in the H1N1 influenza A virus genome.
In vitro vRNA-vRNA interactions in the H1N1 influenza A virus genome.
复制标题
H1N1 甲型流感病毒基因组中的体外 vRNA-vRNA 相互作用。
DOI：
10.1111/1348-0421.12766
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发表时间：
2020
影响因子：
2.6
通讯作者：
Noda T.
中科院分区：
文献类型：
--
作者：
Miyamoto S;Noda T.
甲型流感病毒基因组由八段单链负义病毒RNA(VRNAs)组成。每个vRNA都包含一个中央编码区，其两侧是非编码区。研究表明，在病毒粒子形成时，8个vRNA通过位于每个vRNA的末端编码区和相邻的非编码区的片段特异性包装信号选择性地包装成后代病毒粒子。虽然最近使用下一代测序的研究表明，基因组包装中涉及多个片段间相互作用，但包装信号对片段间相互作用的贡献还不完全清楚。在此，我们利用人工合成的H1N1 WSN(A/WSN/33[H1N1])病毒的全长vRNA和含有包装信号序列的短vRNA进行体外RNA结合分析，发现8个vRNA之间存在15个节段间的相互作用，其中大部分是由3‘-末端和5’-末端介导的。有趣的是，所有8个vRNA都与多个其他vRNA相互作用，其中一些通过各自的3‘-末端和5’-末端区域与不同的vRNA结合。这些体外发现将有助于未来研究选择性基因组包装过程中活体vRNA-vRNA的相互作用。
The genome of influenza A virus consists of eight‐segmented, single‐stranded, negative‐sense viral RNAs (vRNAs). Each vRNA contains a central coding region that is flanked by noncoding regions. It has been shown that upon virion formation, the eight vRNAs are selectively packaged into progeny virions through segment‐specific packaging signals that are located in both the terminal coding regions and adjacent noncoding regions of each vRNA. Although recent studies using next‐generation sequencing suggest that multiple intersegment interactions are involved in genome packaging, contributions of the packaging signals to the intersegment interactions are not fully understood. Herein, using synthesized full‐length vRNAs of H1N1 WSN (A/WSN/33 [H1N1]) virus and short vRNAs containing the packaging signal sequences, we performedin vitroRNA binding assays and identified 15 intersegment interactions among eight vRNAs, most of which were mediated by the 3′‐ and 5′‐terminal regions. Interestingly, all eight vRNAs interacted with multiple other vRNAs, in that some bound to different vRNAs through their respective 3′‐ and 5′‐terminal regions. Thesein vitrofindings would be of use in future studies ofin vivovRNA–vRNA interactions during selective genome packaging.