Analysis of the outer membrane proteome and secretome of Bacteroides fragilis reveals a multiplicity of secretion mechanisms.
Analysis of the outer membrane proteome and secretome of Bacteroides fragilis reveals a multiplicity of secretion mechanisms.
复制标题
对脆弱拟杆菌外膜蛋白质组和分泌组的分析揭示了多种分泌机制。
DOI：
10.1371/journal.pone.0117732
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Bernstein HD
中科院分区：
文献类型：
--
作者：
Wilson MM;Anderson DE;Bernstein HD
脆弱拟杆菌是一种广泛分布于人体肠道菌群的机会致病菌。这种微生物产生的细胞表面分子可能在定植、与其他微生物的交流和致病性中发挥重要作用，但外膜（OM）的蛋白质组成和用于将多肽运输到细胞外空间的机制尚不清楚。本研究采用LC-MS/MS对实验室培养的脆弱芽孢杆菌NCTC 9343的OM蛋白组和分泌组进行了分析。在我们鉴定的229个OM蛋白中，108个被预测为脂蛋白，61个被预测为tonb依赖性转运蛋白。基于它们与编码tonb依赖性转运蛋白的基因的接近性，许多脂蛋白基因可能编码参与营养或小分子摄取的蛋白质。有趣的是，蛋白酶可及性和生物素化实验表明，异常大比例的脂蛋白暴露在细胞表面。我们还鉴定了三种蛋白质，它们是一个新的自转运蛋白家族的成员，多种潜在的I型蛋白质分泌系统，以及似乎是VI型分泌装置的组成部分的蛋白质。分泌组由脂蛋白和其他蛋白质组成，它们可能是假定的I型或VI型分泌系统的底物。我们的蛋白质组学研究表明，脆弱芽孢杆菌在其产生的OM蛋白光谱和其利用的分泌策略范围上与已经得到充分研究的革兰氏阴性菌（如大肠杆菌）有很大不同。
Bacteroides fragilis is a widely distributed member of the human gut microbiome and an opportunistic pathogen. Cell surface molecules produced by this organism likely play important roles in colonization, communication with other microbes, and pathogenicity, but the protein composition of the outer membrane (OM) and the mechanisms used to transport polypeptides into the extracellular space are poorly characterized. Here we used LC-MS/MS to analyze the OM proteome and secretome of B. fragilis NCTC 9343 grown under laboratory conditions. Of the 229 OM proteins that we identified, 108 are predicted to be lipoproteins, and 61 are predicted to be TonB-dependent transporters. Based on their proximity to genes encoding TonB-dependent transporters, many of the lipoprotein genes likely encode proteins involved in nutrient or small molecule uptake. Interestingly, protease accessibility and biotinylation experiments indicated that an unusually large fraction of the lipoproteins are cell-surface exposed. We also identified three proteins that are members of a novel family of autotransporters, multiple potential type I protein secretion systems, and proteins that appear to be components of a type VI secretion apparatus. The secretome consisted of lipoproteins and other proteins that might be substrates of the putative type I or type VI secretion systems. Our proteomic studies show that B. fragilis differs considerably from well-studied Gram-negative bacteria such as Escherichia coli in both the spectrum of OM proteins that it produces and the range of secretion strategies that it utilizes.
登录
查看更多内容
影响因子：
6.4
作者：
Elhenawy W;Debelyy MO;Feldman MF
通讯作者：
Feldman MF
影响因子：
6.4
作者：
Coyne MJ;Zitomersky NL;McGuire AM;Earl AM;Comstock LE
通讯作者：
Comstock LE
影响因子：
3.1
作者：
DeRocco, Amanda J.;Cornelissen, Cynthia Nau
通讯作者：
Cornelissen, Cynthia Nau
影响因子：
3.7
作者：
Blanvillain S;Meyer D;Boulanger A;Lautier M;Guynet C;Denancé N;Vasse J;Lauber E;Arlat M
通讯作者：
Arlat M
影响因子：
3.4
作者：
Flores-Ramirez, Gabriela;Jankovicova, Barbora;Skultety, Ludovit
通讯作者：
Skultety, Ludovit