Advances in collagen cross-link analysis
Advances in collagen cross-link analysis
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DOI：
10.1016/j.ymeth.2008.01.002
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发表时间：
2008-05-01
期刊：
影响因子：
4.8
通讯作者：
Wu, Jiann-Jiu
中科院分区：
文献类型：
--
作者：
Eyre, David R.;Weis, Mary Ann;Wu, Jiann-Jiu
离子阱质谱法和肽特异性抗体的结合应用对胶原交联结构域的分离和结构分析，并举例说明了不同类型的胶原蛋白的结果，重点是骨和软骨。我们强调了这些方法的潜力，以提高对翻译后修饰（例如赖氨酸羟基化和糖基化程度）的重要性的认识，以及末端肽和螺旋交联结构域在调节交联类型和位置方面的首选分子间结合伙伴。(C) 2008爱思唯尔公司版权所有。
The combined application of ion-trap mass spectrometry and peptide-specific antibodies for the isolation and structural analysis of collagen cross-linking domains is illustrated with examples of results from various types of collagen with the emphasis on bone and cartilage. We highlight the potential of such methods to advance knowledge on the importance of post-translational modifications (e.g., degrees of lysine hydroxylation and glycosylation) and preferred intermolecular binding partners for telopeptide and helical cross-linking domains in regulating cross-link type and placement. (C) 2008 Elsevier Inc. All rights reserved.