The PR-1a promoter contains a number of elements that bind GT-1-like nuclear factors with different affinity
The PR-1a promoter contains a number of elements that bind GT-1-like nuclear factors with different affinity
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DOI：
10.1007/bf00049327
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发表时间：
1996-02-01
影响因子：
5.1
通讯作者：
Linthorst, HJM
中科院分区：
文献类型：
--
作者：
Buchel, AS;Molenkamp, R;Linthorst, HJM
利用聚合酶链式反应将烟草PR-1a基因的900bp启动子区域分为8个片段。这些片段被测试了它们与从烟草叶片中分离的核因子结合的能力。条带移位分析表明，除了一个片段外，所有片段都与核蛋白发生了特异性的相互作用。竞争实验表明，相同的核因子以不同的亲和力结合不同的启动子片段。此外，与RBC启动子BoxII的合成四聚体的有效竞争(Green PJ et al.，EMBO J13(1988)4035-4044)表明，GT-L样核因子参与了这些相互作用。此外，与未经处理的植物提取物相比，来自烟草花叶病毒感染烟草的核蛋白制剂显示出GT-1结合活性降低。这些结果将与诱导PR-1a基因表达的关系进行讨论。
The 900 bp promoter region of the tobacco PR-1a gene was divided into eight fragments using PCR. The fragments were tested for their ability to bind to nuclear factors isolated from tobacco leaf. Band shift assays demonstrated that all but one of the fragments specifically interacted with nuclear proteins. From competition experiments it was determined that the same nuclear factors bind various promoter fragments with different affinity. Moreover, efficient competition with a synthetic tetramer of box II of the rbcS promoter (Green PJ et al., EMBO J 13 (1988) 4035-4044) indicated that GT-l-like nuclear factors are involved in these interactions. Furthermore, in comparison to extracts from untreated plants, nuclear protein preparations from tobacco mosaic virus-infected tobacco showed a reduced GT-1 binding activity. These results will be discussed in relation to induced PR-1a gene expression.