HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROCHEMICAL ASSAY FOR MONITORING THE FORMATION OF 8-OXO-7,8-DIHYDROADENINE AND ITS RELATED 2'-DEOXYRIBONUCLEOSIDE
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROCHEMICAL ASSAY FOR MONITORING THE FORMATION OF 8-OXO-7,8-DIHYDROADENINE AND ITS RELATED 2'-DEOXYRIBONUCLEOSIDE
复制标题
DOI：
10.1080/01483919008049222
复制
发表时间：
1990-01-01
期刊：
影响因子：
--
通讯作者：
CADET, J
中科院分区：
文献类型：
--
作者：
BERGER, M;ANSELMINO, C;CADET, J
开发了一种结合使用反相高效液相色谱和电化学检测的灵敏方法来监测8-氧代-7,8-二氢腺嘌呤和8-氧代-7,8-二氢-2''-脱氧腺苷的形成。在氧化电位 850 mV 的优化检测条件下，两种化合物的检测限接近 0.2 ng。该测定可以定量测量暴露于低至 2 戈瑞 (Gy) 剂量的电离辐射的腺嘌呤和 2''-脱氧腺苷水溶液中的羟基化嘌呤成分。
A sensitive method involving the combined use of reversed-phase high-performance liquid chromatography and electrochemical detection has been developed for monitoring the formation of 8-oxo-7,8-dihydroadenine and 8-oxo-7,8-dihydro-2''-deoxyadenosine. The limit of detection for both compounds is close to 0.2 ng under optimized conditions of detection at an oxidation potential of 850 mV. This assay allows the quantitative measurement of the hydroxylated purine components in aqueous solutions of adenine and 2''-deoxyadenosine exposed to a dose of ionizing radiation as low as 2 Gray (Gy).