Pathologic and virologic findings in mid-gestational porcine foetuses after experimental inoculation with PCV2a or PCV2b
Pathologic and virologic findings in mid-gestational porcine foetuses after experimental inoculation with PCV2a or PCV2b
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DOI：
10.1016/j.vetmic.2010.03.017
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发表时间：
2010-09-28
影响因子：
3.3
通讯作者：
Nauwynck, H. J.
中科院分区：
文献类型：
--
作者：
Saha, D.;Lefebvre, D. J.;Nauwynck, H. J.
猪圆环病毒2型（PCV 2）有两种主要的基因型：PCV 2a和PCV 2b。以前的研究主要是使用PCV 2a来实验性地复制母猪的繁殖失败。本研究旨在确定55天大免疫缺陷猪胎儿手术接种PCV 2a或PCV 2b的临床和病毒学结果。12只胎仔接种PCV 2：3只接种断奶后多系统衰竭综合征（PMWS）相关PCV 2a Stoon-1010株，3只接种生殖衰竭相关PCV 2a 1121株，3只接种PMWS相关PCV 2b 48285株，3只接种猪皮炎和肾病综合征相关PCV 2b 1147株。用细胞培养基模拟接种4只胎仔。在接种后21天，11/12只接种PCV 2的胎仔出现水肿并有扩张的阴茎，而1只胎仔外观正常。所有接种PCV 2的胎仔均出现内脏出血和充血以及肝脏肿大。在所有接种PCV 2的胎仔中均发现高PCV 2滴度（>10（4.5）TCID（50）/g组织），尤其是在心脏、脾脏和肝脏中。在心脏中观察到大量PCV 2感染细胞（>1000个感染细胞/10 mm 2组织）。衣壳基因的PCR和DNA测序分别从接种PCV 2a和PCV 2b的胎仔中回收了纯PCV 2a和纯PCV 2b序列。所有模拟接种胎仔和其余胎仔外观正常，病毒滴定和间接免疫荧光染色均为PCV 2阴性。本研究表明，PCV 2a和PCV 2b在55日龄免疫缺陷猪胎仔的器官中诱导相似的大体病理学病变并复制至相似的高滴度。(c)2010爱思唯尔有限公司版权所有。
Two major genotypes of porcine circovirus type 2 (PCV2) have been described: PCV2a and PCV2b. Previous studies mainly used PCV2a to experimentally reproduce reproductive failure in sows. This study aims to determine the clinical and virological outcome of surgical inoculation of 55-day-old immuno-incompetent porcine foetuses with PCV2a or PCV2b. Twelve foetuses were inoculated with PCV2: three with the post-weaning multisystemic wasting syndrome (PMWS)-associated PCV2a strain Stoon-1010, three with the reproductive failure-associated PCV2a strain 1121, three with the PMWS-associated PCV2b strain 48285 and three with the porcine dermatitis and nephropathy syndrome-associated PCV2b strain 1147. Four foetuses were mock-inoculated with cell culture medium. At 21 days post-inoculation eleven out of twelve PCV2-inoculated foetuses were oedematous and had distended abdomens, whereas one had a normal external appearance. All PCV2-inoculated foetuses had haemorrhages and congestion in internal organs and an enlarged liver. High PCV2 titres (>10(4.5)TCID(50)/g tissue) were found in all PCV2-inoculated foetuses, especially in the heart, spleen and liver. High numbers of PCV2-infected cells (>1000 infected cells/10 mm(2) tissue) were observed in the hearts. PCR and DNA sequencing of the capsid gene recovered pure PCV2a and pure PCV2b sequences from PCV2a- and PCV2b-inoculated foetuses, respectively. All mock-inoculated and the remaining foetuses were normal in appearance and were PCV2 negative in virus titrations and indirect immunofluorescence stainings. The present study shows that PCV2a and PCV2b induce similar gross pathological lesions and replicate to similar high titres in organs of 55-day-old immuno-incompetent porcine foetuses. (c) 2010 Elsevier B.V. All rights reserved.