Periodic cyclin-Cdk activity entrains an autonomous Cdc14 release oscillator.
Periodic cyclin-Cdk activity entrains an autonomous Cdc14 release oscillator.
复制标题
DOI：
10.1016/j.cell.2010.03.021
复制
发表时间：
2010-04-16
期刊：
影响因子：
64.5
通讯作者：
Cross FR
中科院分区：
文献类型：
--
作者：
Lu Y;Cross FR
周期蛋白依赖性激酶（Cdk）活性的一个振荡主要由周期蛋白的周期性合成和破坏驱动，与单个完整的真核细胞分裂周期紧密耦合。不同的细胞周期过程中的不同步骤的紧密联系，以Cdk活性已被提出来解释这种耦合。在这里，我们展示了一个内在的振荡模块控制核仁释放和重新螯合的Cdc 14磷酸酶，这是必不可少的有丝分裂退出芽殖酵母。我们发现，这Cdc 14释放振荡器的功能在恒定的和生理的细胞周期蛋白-Cdk水平，因此是独立的Cdk振荡器。然而，释放振荡器的频率由细胞周期蛋白-Cdk活性调节。这一观察结果及其机制表明，本质上自主的Cdc 14释放周期通过野生型细胞中的Cdk振荡器的夹带而锁定在每个细胞周期一次。这一概念可能对真核细胞周期控制的结构和进化具有广泛的意义。
One oscillation of Cyclin-dependent kinase (Cdk) activity, largely driven by periodic synthesis and destruction of cyclins, is tightly coupled to a single complete eukaryotic cell division cycle. Tight linkage of different steps in diverse cell cycle processes to Cdk activity has been proposed to explain this coupling. Here we demonstrate an intrinsically oscillatory module controlling nucleolar release and re-sequestration of the Cdc14 phosphatase, which is essential for mitotic exit in budding yeast. We find that this Cdc14 release oscillator functions at constant and physiological cyclin-Cdk levels, and is therefore independent of the Cdk oscillator. However, the frequency of the release oscillator is regulated by cyclin-Cdk activity. This observation together with its mechanism suggests that the intrinsically autonomous Cdc14 release cycles are locked at once-per-cell-cycle through entrainment by the Cdk oscillator in wild type cells. This concept may have broad implications for the structure and evolution of eukaryotic cell cycle control.