Comparison of inflammatory changes caused by Porphyromonas gingivalis with distinct fimA genotypes in a mouse abscess model
Comparison of inflammatory changes caused by Porphyromonas gingivalis with distinct fimA genotypes in a mouse abscess model
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DOI：
10.1111/j.0902-0055.2004.00133.x
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发表时间：
2004-06-01
影响因子：
--
通讯作者：
Amano, A
中科院分区：
文献类型：
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作者：
Nakano, K;Kuboniwa, M;Amano, A
牙龈卟啉单胞菌的fimA基因编码菌毛蛋白（fimbrillin），目前已将其分为I-V型和Ib型6种基因型。基因型变异先前被认为与一般人群中成人牙周炎的严重程度有关。在这项研究中，我们比较了由细菌感染引起的炎症变化，以研究小鼠脓肿模型中不同fimA菌株之间的致病异质性。将代表六种fimA类型的13种牙龈卟啉单胞菌菌株的细菌悬液皮下注射到雌性BALB/c小鼠中，并测定血清唾液酸浓度作为定量宿主炎症参数。II型fimA微生物引起血清唾液酸的最显著诱导，以及其他感染症状，其次是Ib、IV和V型。相反，I型和III型引起较弱的炎症变化。此外，II型菌株的fimA突变体明显失去了它们的感染能力。这些发现表明fimA基因型变异影响牙龈卟啉单胞菌毒力的表达。
The fimA gene of Porphyromonas gingivalis, encoding fimbrillin (a subunit protein of fimbriae) has been classified into six genotypes (types I-V and Ib). The genotypic variation was previously suggested to be related to the severity of adult periodontitis in the general population. In this study, we compared inflammatory changes caused by bacterial infection to study pathogenic heterogeneity among the different fimA strains in a mouse abscess model. Bacterial suspensions of 13 P. gingivalis strains representing the six fimA types were subcutaneously injected into female BALB/c mice, and serum sialic acid concentrations were assayed as a quantitative host inflammatory parameter. Type II fimA organisms caused the most significant induction of serum sialic acid, as well as other infectious symptoms, followed by types Ib, IV and V. In contrast, types I and III caused weak inflammatory changes. In addition, fimA mutants of type II strains clearly lost their infectious ability. These findings suggest that fimA genotypic variation affects expression of P. gingivalis virulence.